Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.242 extracted from

  • Chaiyen, P.; Ballou, D.P.; Massey, V.
    Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (1997), Proc. Natl. Acad. Sci. USA, 94, 7233-7238.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.242

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 5-hydroxynicotinic acid recombinant enzyme Pseudomonas sp.
0.068
-
 5-hydroxynicotinic acid wild-type enzyme Pseudomonas sp.
0.126
-
 O2 wild-type enzyme Pseudomonas sp.
0.148
-
 O2 recombinant enzyme Pseudomonas sp.
0.18
-
 NADH recombinant enzyme Pseudomonas sp.
0.205
-
 NADH wild-type enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41700
-
 4 * 41700, calculation from nucleotide sequence Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. O08453 MA-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2
-
 Pseudomonas sp. 2-(acetamidomethylene)succinate + NAD(P)+
-
 ?
5-hydroxynicotinic acid + NADH + O2
-
 Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41700, calculation from nucleotide sequence Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD contains one FAD per subunit, tetrameric enzyme Pseudomonas sp.
NADH
-
 Pseudomonas sp.