Literature summary for 1.14.13.24 extracted from

Srisook, T.; Vongsetskul, T.; Sucharitakul, J.; Chaiyen, P.; Tangboriboonrat, P.
Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers A novel heterogeneous catalyst (2014), React. Funct. Polym., 82, 41-46 .

No PubMed abstract available

Search for more literature for 1.14.13.24

General Stability

General Stability	Organism
immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers can tolerate the changes in temperature and pH better than the free enzyme can. The immobilized enzyme can be reused at least 10 times with more than 60% of the original activity at pH 8 and 25°C. The 3-hydroxybenzoate 6-hydroxylase fibers are potentially useful as a heterogeneous catalyst under the conditions in which free enzyme can not endure.	Rhodococcus jostii

Organism

immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers can tolerate the changes in temperature and pH better than the free enzyme can. The immobilized enzyme can be reused at least 10 times with more than 60% of the original activity at pH 8 and 25°C. The 3-hydroxybenzoate 6-hydroxylase fibers are potentially useful as a heterogeneous catalyst under the conditions in which free enzyme can not endure.

Rhodococcus jostii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.046	-	3-hydroxybenzoate	pH 8, 25°C, native enzyme	Rhodococcus jostii
0.142	-	3-hydroxybenzoate	pH 8, 25°C, enzyme immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers	Rhodococcus jostii
2.5	-	NADH	pH 8, 25°C, native enzyme	Rhodococcus jostii

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus jostii	Q0SFK6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxybenzoate + NADH + H+ + O2	-	Rhodococcus jostii	2,5-dihydroxybenzoate + NAD+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
3HB6H	-	Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0178	-	3-hydroxybenzoate	pH 8, 25°C, enzyme immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers	Rhodococcus jostii
21	-	3-hydroxybenzoate	pH 8, 25°C, native enzyme	Rhodococcus jostii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	soluble enzyme	Rhodococcus jostii
8	-	enzyme immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers	Rhodococcus jostii

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	8	at pH 5.5-8, the residual activity of soluble enzyme is less than that of the immobilized one	Rhodococcus jostii
9	10	at pH 9 and 10, the enzyme activity of of the soluble enzyme is higher than that of the immobilized enzyme	Rhodococcus jostii