Literature summary for 1.14.13.24 extracted from

Montersino, S.; Te Poele, E.; Orru, R.; Westphal, A.H.; Barendregt, A.; Heck, A.J.R.; van der Geize, R.; Dijkhuizen, L.; Mattevi, A.; van Berkel, W.J.H.
3-Hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1 contains a phosphatidylinositol cofactor (2017), Front. Microbiol., 8, 1110 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Rhodococcus jostii RHA#2	Rhodococcus jostii

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus jostii	Q0SFK6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodococcus jostii

Synonyms

Synonyms	Comment	Organism
3-hydroxybenzoate 6-hydroxylase	-	Rhodococcus jostii
3HB6H	-	Rhodococcus jostii

Cofactor

Cofactor	Comment	Organism	Structure
FAD	flavoprotein	Rhodococcus jostii
additional information	the enzyme harbors a phospholipid ligand. The purified enzyme obtained from expressing the gene encoding 3HB6H from Rhodococcus jostii RHA1 in the host Escherichia coli contains a mixture of phosphatidylglycerol and phosphatidylethanolamine, which are the major constituents of the cytoplasmic membrane of Escherichia coli. The enzyme that is produced in the host Rhodococcus jostii RHA#2 by employing a newly developed actinomycete expression system, contains phosphatidylinositol, which is a specific constituent of actinomycete membranes. The lipid cofactor stabilizes monomer-monomer contact	Rhodococcus jostii

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes a key step in the catabolism of lignin-derived aromatic compounds	Rhodococcus jostii

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Release 2023.1 (January 2023)