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Literature summary for 1.14.13.24 extracted from

  • Liu, D.Q.; Liu, H.; Gao, X.L.; Leak, D.J.; Zhou, N.Y.
    Arg169 is essential for catalytic activity of 3-hydroxybenzoate 6-hydroxylase from Klebsiella pneumoniae M5a1 (2005), Microbiol. Res., 160, 53-59.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mhbM, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha Klebsiella oxytoca

Protein Variants

Protein Variants Comment Organism
R169E site-directed mutagenesis, inactive mutant Klebsiella oxytoca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, about, SDS-PAGE Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + NADH + O2 Klebsiella oxytoca
-
2,5-dihydroxybenzoate + NAD+ + H2O i.e. gentisate ?

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca Q5EXK1 gene mhbM
-

Reaction

Reaction Comment Organism Reaction ID
3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O Arg169 is essential for catalytic activity Klebsiella oxytoca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
recombinant wild-type enzyme Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NADH + O2
-
Klebsiella oxytoca 2,5-dihydroxybenzoate + NAD+ + H2O i.e. gentisate ?

Subunits

Subunits Comment Organism
? x * 44000, about, SDS-PAGE Klebsiella oxytoca
More Ser166 and Arg169 form the highly conserved SXXR motif important for structure and function of the enzyme Klebsiella oxytoca

Synonyms

Synonyms Comment Organism
3-hydroxybenzoate 6-hydroxylase
-
Klebsiella oxytoca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Klebsiella oxytoca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Klebsiella oxytoca

Cofactor

Cofactor Comment Organism Structure
FAD
-
Klebsiella oxytoca
NADH
-
Klebsiella oxytoca