General Stability | Organism |
---|---|
complete inactivation at room temperature without glycerol after 18 h | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol | Klebsiella pneumoniae | |
Fe2+ | inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol | Klebsiella pneumoniae | |
Hg2+ | inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADPH | - |
Klebsiella pneumoniae | |
0.07 | - |
NADH | - |
Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
gel filtration | Klebsiella pneumoniae |
42000 | - |
1 * 42000, SDS-PAGE | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae M5a1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.42 | - |
- |
Klebsiella pneumoniae |
Storage Stability | Organism |
---|---|
4°C, optimal storage in 50 mM phosphate or Tris buffer pH 8 with 10% glycerol | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + O2 | - |
Klebsiella pneumoniae | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
3-hydroxybenzoate + NADH + O2 | - |
Klebsiella pneumoniae M5a1 | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
3-hydroxybenzoate + NADPH + H+ + O2 | - |
Klebsiella pneumoniae | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
r | |
3-hydroxybenzoate + NADPH + H+ + O2 | - |
Klebsiella pneumoniae M5a1 | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | no activity below 15°C and above 40°C | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | no activity below pH 6.5 and above pH 11 | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | in phosphate and Tris-HCl buffer | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Klebsiella pneumoniae | |
NADPH | NADH and NADPH utilized with similar efficiencies | Klebsiella pneumoniae |