BRENDA - Enzyme Database
show all sequences of 1.14.13.238

Identification of dimethylamine monooxygenase in marine bacteria reveals a metabolic bottleneck in the methylated amine degradation pathway

Lidbury, I.; Mausz, M.A.; Scanlan, D.J.; Chen, Y.; ISME J. 11, 1592-1601 (2017)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BLR(DE3)pLysS cells
Ruegeria pomeroyi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethylamine + NADPH + H+ + O2
Ruegeria pomeroyi
-
methylamine + formaldehyde + NADP+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Ruegeria pomeroyi
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dimethylamine + NADPH + H+ + O2
-
742712
Ruegeria pomeroyi
methylamine + formaldehyde + NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
dmmABC
-
Ruegeria pomeroyi
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Ruegeria pomeroyi
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BLR(DE3)pLysS cells
Ruegeria pomeroyi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Ruegeria pomeroyi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethylamine + NADPH + H+ + O2
Ruegeria pomeroyi
-
methylamine + formaldehyde + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dimethylamine + NADPH + H+ + O2
-
742712
Ruegeria pomeroyi
methylamine + formaldehyde + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.238
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742712
Lidbury
Identification of dimethylami ...
Ruegeria pomeroyi
ISME J.
11
1592-1601
2017
-
-
1
-
-
-
-
-
-
-
-
1
-
8
-
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-
-
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1
-
1
-
-
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-
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-
1
-
-
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-
1
1
-
-
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742736
Hawkins
-
Intrasubstituent isotope effe ...
Aminobacter aminovorans
J. Am. Chem. Soc.
114
3547-3549
1992
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742819
Alberta
Spectroscopic characterizatio ...
Aminobacter aminovorans
J. Biol. Chem.
264
20467-20473
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
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1
-
1
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-
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2
-
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2
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-
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-
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-
1
-
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1
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743014
Green
Subcellular localization and ...
Cyberlindnera jadinii, Cyberlindnera jadinii WY-3
J. Gen. Microbiol.
130
2577-2588
1984
-
-
-
-
-
-
16
19
1
-
-
-
-
2
-
-
1
-
-
-
-
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23
-
1
-
-
1
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-
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-
4
-
-
-
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4
-
-
-
-
16
-
19
1
-
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-
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-
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1
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-
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23
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
741840
Eady
Microbial oxidation of amines ...
Aminobacter aminovorans
Biochem. J.
125
449-459
1971
-
-
-
-
-
-
1
9
-
-
-
-
-
1
-
-
1
-
-
1
-
-
9
-
-
-
-
-
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1
-
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2
-
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-
-
-
-
2
-
-
-
-
1
-
9
-
-
-
-
-
-
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1
-
1
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9
-
-
-
-
-
1
-
-
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-
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-
-
-
-
741839
Eady
Bacterial oxidation of dimeth ...
Aminobacter aminovorans
Biochem. J.
111
37P-38P
1969
-
-
-
-
-
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2
-
-
-
-
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-
1
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1
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1
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2
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1
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1
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2
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2
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2
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1
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1
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2
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-
-
1
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-
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-