Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dimethylamine | substrate inhibition at concentrations higher than 1 mM | Aminobacter aminovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
NADH | at pH 6.8 and 28°C | Aminobacter aminovorans | |
0.0132 | - |
NADPH | at pH 6.8 and 28°C | Aminobacter aminovorans | |
0.041 | - |
ethylamine | at pH 6.8 and 28°C | Aminobacter aminovorans | |
0.058 | - |
Dimethylamine | at pH 6.8 and 28°C | Aminobacter aminovorans | |
0.345 | - |
Diethylamine | at pH 6.8 and 28°C | Aminobacter aminovorans | |
0.63 | - |
methyl-n-propylamine | at pH 6.8 and 28°C | Aminobacter aminovorans | |
0.75 | - |
N-Methylethanolamine | at pH 6.8 and 28°C | Aminobacter aminovorans | |
4.16 | - |
ethyl-n-propylamine | at pH 6.8 and 28°C | Aminobacter aminovorans | |
5.4 | - |
n-butylmethylamine | at pH 6.8 and 28°C | Aminobacter aminovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aminobacter aminovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Aminobacter aminovorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:ethylamine-grown cell | - |
Aminobacter aminovorans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethylamine + NADH + H+ + O2 | 40% activity compared to dimethylamine | Aminobacter aminovorans | ethylamine + acetaldehyde + NAD+ + H2O | - |
? | |
dimethylamine + NADH + H+ + O2 | 100% activity | Aminobacter aminovorans | methylamine + formaldehyde + NAD+ + H2O | - |
? | |
dimethylamine + NADPH + H+ + O2 | good substrate | Aminobacter aminovorans | methylamine + formaldehyde + NADP+ + H2O | - |
? | |
ethyl-n-propylamine + NADH + H+ + O2 | 22% activity compared to dimethylamine | Aminobacter aminovorans | ? | - |
? | |
ethylamine + NADH + H+ + O2 | 65% activity compared to dimethylamine | Aminobacter aminovorans | ? | - |
? | |
methyl-n-propylamine + NADH + H+ + O2 | 87% activity compared to dimethylamine | Aminobacter aminovorans | ? | - |
? | |
additional information | no activity with methylamine, ethylamine, n-propylamine, ethanolamine, aniline, 1,2-diaminopropane, 1,3-diaminopropane, di-isopropylamine, di-n-propylamine, di-isobutylamine, di-n-butylamine, diethanolamine, methyl-n-octylamine, N-methylaniline, benzylmethylamine, putrescine, spermidine, piperidine, sarcosine, N-methylglutamate, noradrenaline, trimethylamine, triethylamine, N,N-dimethylaniline, tetramethylammonium chloride, and tetraethylammonium chloride | Aminobacter aminovorans | ? | - |
? | |
n-butylmethylamine + NADH + H+ + O2 | 54% activity compared to dimethylamine | Aminobacter aminovorans | ? | - |
? | |
n-methylethanolamine + NADH + H+ + O2 | 19% activity compared to dimethylamine | Aminobacter aminovorans | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aminobacter aminovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Aminobacter aminovorans | |
NADPH | - |
Aminobacter aminovorans |