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Literature summary for 1.14.13.238 extracted from

  • Eady, R.; Jarman, T.; Large, P.
    Microbial oxidation of amines. Partial purification of a mixed-function secondary-amine oxidase system from Pseudomonas aminovorans that contains an enzymically active cytochrome-P-420-type haemoprotein (1971), Biochem. J., 125, 449-459 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Dimethylamine substrate inhibition at concentrations higher than 1 mM Aminobacter aminovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
NADH at pH 6.8 and 28°C Aminobacter aminovorans
0.0132
-
NADPH at pH 6.8 and 28°C Aminobacter aminovorans
0.041
-
ethylamine at pH 6.8 and 28°C Aminobacter aminovorans
0.058
-
Dimethylamine at pH 6.8 and 28°C Aminobacter aminovorans
0.345
-
Diethylamine at pH 6.8 and 28°C Aminobacter aminovorans
0.63
-
methyl-n-propylamine at pH 6.8 and 28°C Aminobacter aminovorans
0.75
-
N-Methylethanolamine at pH 6.8 and 28°C Aminobacter aminovorans
4.16
-
ethyl-n-propylamine at pH 6.8 and 28°C Aminobacter aminovorans
5.4
-
n-butylmethylamine at pH 6.8 and 28°C Aminobacter aminovorans

Organism

Organism UniProt Comment Textmining
Aminobacter aminovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-cellulose column chromatography Aminobacter aminovorans

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ethylamine-grown cell
-
Aminobacter aminovorans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethylamine + NADH + H+ + O2 40% activity compared to dimethylamine Aminobacter aminovorans ethylamine + acetaldehyde + NAD+ + H2O
-
?
dimethylamine + NADH + H+ + O2 100% activity Aminobacter aminovorans methylamine + formaldehyde + NAD+ + H2O
-
?
dimethylamine + NADPH + H+ + O2 good substrate Aminobacter aminovorans methylamine + formaldehyde + NADP+ + H2O
-
?
ethyl-n-propylamine + NADH + H+ + O2 22% activity compared to dimethylamine Aminobacter aminovorans ?
-
?
ethylamine + NADH + H+ + O2 65% activity compared to dimethylamine Aminobacter aminovorans ?
-
?
methyl-n-propylamine + NADH + H+ + O2 87% activity compared to dimethylamine Aminobacter aminovorans ?
-
?
additional information no activity with methylamine, ethylamine, n-propylamine, ethanolamine, aniline, 1,2-diaminopropane, 1,3-diaminopropane, di-isopropylamine, di-n-propylamine, di-isobutylamine, di-n-butylamine, diethanolamine, methyl-n-octylamine, N-methylaniline, benzylmethylamine, putrescine, spermidine, piperidine, sarcosine, N-methylglutamate, noradrenaline, trimethylamine, triethylamine, N,N-dimethylaniline, tetramethylammonium chloride, and tetraethylammonium chloride Aminobacter aminovorans ?
-
?
n-butylmethylamine + NADH + H+ + O2 54% activity compared to dimethylamine Aminobacter aminovorans ?
-
?
n-methylethanolamine + NADH + H+ + O2 19% activity compared to dimethylamine Aminobacter aminovorans ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aminobacter aminovorans

Cofactor

Cofactor Comment Organism Structure
NADH
-
Aminobacter aminovorans
NADPH
-
Aminobacter aminovorans