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Literature summary for 1.14.13.236 extracted from
- Cloning of toluene 4-monooxygenase genes and application of two-phase system to the production of the anticancer agent, indirubin (2015), Mol. Biotechnol., 57, 720-726.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | development of a two-phase (dioctyl phthalate/aqueous medium) culture system to obtain maximal indirubin from indole. A 50% (v/v) dioctyl phthalate two-phase system using tryptophan medium containing 3 mM cysteine, 5 mM indole, and 1 mM isatin yields 102.4 mg/l of indirubin with no conversion of indole to indigo | Pseudomonas sp. M4 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas sp. M4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. M4 | A0A0D3QM77 and A0A0D3QME2 and A0A0D3QLU4 and A0A0D3QM47 and A0A0D3QMJ7 and A0A0D3QM80 | A0A0D3QM77 i.e. subunit TmoA, A0A0D3QME2 i.e. subunit TmoB, A0A0D3QLU4 i.e. subunit TmoC, A0A0D3QM47 i.e. subunit TmD, A0A0D3QMJ7 i.e. subunit TmoE, A0A0D3QM80 i.e. subunit TmoF | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 indole + 3 NADH + 3 H+ + 3 O2 | - |
Pseudomonas sp. M4 | indirubin + 3 NAD+ + 3 H2O | reaction via C-3 oxidation to indoxyl, oxidation to isatin, and recombinantion of isatin and indoxyl to indirubin | ? |  |
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