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Literature summary for 1.14.13.236 extracted from

  • Brouk, M.; Derry, N.; Shainsky, J.; Zelas, Z.; Boyko, Y.; Dabush, K.; Fishman, A.
    The influence of key residues in the tunnel entrance and the active site on activity and selectivity of toluene-4-monooxygenase (2010), J. Mol. Catal. B, 66, 72-80.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
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Pseudomonas mendocina

Protein Variants

Protein Variants Comment Organism
D285A mutation in subunit TmoA, 2.7fold increase in activity with 2-phenylethanol Pseudomonas mendocina
D285C mutation in subunit TmoA, 4fold increase in activity with 2-phenylethanol Pseudomonas mendocina
D285I mutation in subunit TmoA, 6.6fold increase in activity with 2-phenylethanol Pseudomonas mendocina
D285L mutation in subunit TmoA, 5.4fold increase in activity with 2-phenylethanol Pseudomonas mendocina
D285P mutation in subunit TmoA, 3.3fold increase in activity with 2-phenylethanol Pseudomonas mendocina
D285Q mutation in subunit TmoA, 10.5fold increase in activity with 2-phenylethanol Pseudomonas mendocina
D285S mutation in subunit TmoA, 70% of wild-type activity Pseudomonas mendocina
D285Y mutation in subunit TmoA, 3fold increase in activity with 2-phenylethanol Pseudomonas mendocina
I100A mutation in subunit TmoA, 35fold increase in activity with 2-phenylethanol Pseudomonas mendocina
I100A/D285I mutation in subunit TmoA, 52fold increase in activity with 2-phenylethanol Pseudomonas mendocina
I100A/D285Q mutation in subunit TmoA, 85fold increase in activity with 2-phenylethanol Pseudomonas mendocina
I100G mutation in subunit TmoA, 11fold increase in activity with methyl p-tolyl sulfide Pseudomonas mendocina
I100G/D285I mutation in subunit TmoA, 14.1fold increase in activity with methyl p-tolyl sulfide Pseudomonas mendocina
I100L mutation in subunit TmoA, 0.9fold decrease in activity with styrene Pseudomonas mendocina
I100L/D285S mutation in subunit TmoA, 1.4fold increase in activity with styrene Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF
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Pseudomonas mendocina KR1 Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl p-tolyl sulfide + NADH + H+ + O2
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Pseudomonas mendocina methyl p-tolyl sulfoxide + NAD+ + H2O
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?
methyl p-tolyl sulfide + NADH + H+ + O2
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Pseudomonas mendocina KR1 methyl p-tolyl sulfoxide + NAD+ + H2O
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?
phenylethanol + NADH + H+ + O2
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Pseudomonas mendocina m-tyrosol + p-tyrosol + NAD+ + H2O 63% m-tyrosol + 37% p-tyrosol ?
phenylethanol + NADH + H+ + O2
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Pseudomonas mendocina KR1 m-tyrosol + p-tyrosol + NAD+ + H2O 63% m-tyrosol + 37% p-tyrosol ?
styrene + NADH + H+ + O2
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Pseudomonas mendocina styrene epoxide + NAD+ + H2O
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?
styrene + NADH + H+ + O2
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Pseudomonas mendocina KR1 styrene epoxide + NAD+ + H2O
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?