Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas mendocina |
Protein Variants | Comment | Organism |
---|---|---|
D285A | mutation in subunit TmoA, 2.7fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
D285C | mutation in subunit TmoA, 4fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
D285I | mutation in subunit TmoA, 6.6fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
D285L | mutation in subunit TmoA, 5.4fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
D285P | mutation in subunit TmoA, 3.3fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
D285Q | mutation in subunit TmoA, 10.5fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
D285S | mutation in subunit TmoA, 70% of wild-type activity | Pseudomonas mendocina |
D285Y mutation in subunit TmoA, | 3fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
I100A | mutation in subunit TmoA, 35fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
I100A/D285I | mutation in subunit TmoA, 52fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
I100A/D285Q | mutation in subunit TmoA, 85fold increase in activity with 2-phenylethanol | Pseudomonas mendocina |
I100G | mutation in subunit TmoA, 11fold increase in activity with methyl p-tolyl sulfide | Pseudomonas mendocina |
I100G/D285I | mutation in subunit TmoA, 14.1fold increase in activity with methyl p-tolyl sulfide | Pseudomonas mendocina |
I100L | mutation in subunit TmoA, 0.9fold decrease in activity with styrene | Pseudomonas mendocina |
I100L/D285S | mutation in subunit TmoA, 1.4fold increase in activity with styrene | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 | Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF | - |
Pseudomonas mendocina KR1 | Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 | Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl p-tolyl sulfide + NADH + H+ + O2 | - |
Pseudomonas mendocina | methyl p-tolyl sulfoxide + NAD+ + H2O | - |
? | |
methyl p-tolyl sulfide + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | methyl p-tolyl sulfoxide + NAD+ + H2O | - |
? | |
phenylethanol + NADH + H+ + O2 | - |
Pseudomonas mendocina | m-tyrosol + p-tyrosol + NAD+ + H2O | 63% m-tyrosol + 37% p-tyrosol | ? | |
phenylethanol + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | m-tyrosol + p-tyrosol + NAD+ + H2O | 63% m-tyrosol + 37% p-tyrosol | ? | |
styrene + NADH + H+ + O2 | - |
Pseudomonas mendocina | styrene epoxide + NAD+ + H2O | - |
? | |
styrene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | styrene epoxide + NAD+ + H2O | - |
? |