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Literature summary for 1.14.13.236 extracted from

  • Tao, Y.; Fishman, A.; Bentley, W.E.; Wood, T.K.
    Altering toluene 4-monooxygenase by active-site engineering for the synthesis of 3-methoxycatechol, methoxyhydroquinone, and methylhydroquinone (2004), J. Bacteriol., 186, 4705-4713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.236

Cloned(Commentary)

Cloned (Comment) Organism
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 Pseudomonas mendocina

Protein Variants

Protein Variants Comment Organism
G103A/A107S mutation in subunit TmoA, produces 3-methylcatechol (98%) from o-cresol twofold faster and produces 3-methoxycatechol (82%) from 1mM o-methoxyphenol seven times faster than the wild-type Pseudomonas mendocina
G103S mutation in subunit TmoA, produces 40fold more methoxyhydroquinone from o-methoxyphenol than the wild-type Pseudomonas mendocina
G103S/A107T mutation in subunit TmoA, produces methylhydroquinone (92%) from o-cresol fourfold faster than wild-type Pseudomonas mendocina
I100L mutation in subunit TmoA, produces 3-methoxycatechol from o-methoxyphenol four times faster than wild-type Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF
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Pseudomonas mendocina KR1 Q6Q8Q7 and Q6Q8Q6 and Q6Q8Q5 and Q6Q8Q4 and Q6Q8Q3 and Q6Q8Q2 Q6Q8Q7 i.e. subunit TmoA, Q6Q8Q6 i.e. subunit TmoB, Q6Q8Q5 i.e. subunit TmoC, Q6Q8Q4 i.e. subunit TmoD, Q6Q8Q3 i.e. subunit TmoE, Q6Q8Q2 i.e. subunit TmoF
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
m-cresol + NADH + H+ + O2
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 Pseudomonas mendocina 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
m-cresol + NADH + H+ + O2
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 Pseudomonas mendocina KR1 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
o-cresol + NADH + H+ + O2
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 Pseudomonas mendocina 3-methylcatechol + NAD+ + H2O 91% 3-methylcatechol + 9% methylhydroquinone ?
o-cresol + NADH + H+ + O2
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 Pseudomonas mendocina KR1 3-methylcatechol + NAD+ + H2O 91% 3-methylcatechol + 9% methylhydroquinone ?
o-methoxyphenol + NADH + H+ + O2
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 Pseudomonas mendocina 4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O 87% 4-methoxyresorcinol, 11% 3-methoxycatechol, and 2% methoxyhydroquinone ?
o-methoxyphenol + NADH + H+ + O2
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 Pseudomonas mendocina KR1 4-methoxyresorcinol + 3-methoxycatechol + NAD+ + H2O 87% 4-methoxyresorcinol, 11% 3-methoxycatechol, and 2% methoxyhydroquinone ?
p-cresol + NADH + H+ + O2
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 Pseudomonas mendocina 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
p-cresol + NADH + H+ + O2
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 Pseudomonas mendocina KR1 4-methylcatechol + NAD+ + H2O 100% 4-methylcatechol ?
toluene + NADH + H+ + O2
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 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96% 4-methylphenol + 3% 3-methylphenol ?
toluene + NADH + H+ + O2
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 Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 96% 4-methylphenol + 3% 3-methylphenol ?