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Literature summary for 1.14.13.236 extracted from

  • Whited, G.M.; Gibson, D.T.
    Toluene-4-monooxygenase, a three-component enzyme system that catalyzes the oxidation of toluene to p-cresol in Pseudomonas mendocina KR1 (1991), J. Bacteriol., 173, 3010-3016.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q00456 and Q00457 and Q00460 and Q00458 and Q00459 Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively, Q00458 i.e. Rieske-type ferredoxin T4MOC, Q00459 i.e. effector protein T4MOD
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Pseudomonas mendocina KR1 Q00456 and Q00457 and Q00460 and Q00458 and Q00459 Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively, Q00458 i.e. Rieske-type ferredoxin T4MOC, Q00459 i.e. effector protein T4MOD
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Purification (Commentary)

Purification (Comment) Organism
partial purification from toluene-grown cells Pseudomonas mendocina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information experiments with p-deuterotoluene lead to the isolation of p-cresol which retains 68% of the deuterium initially present in the parent molecule. When incubated with toluene in the presence of 1802, the oxygen in p-cresol is derived from molecular oxygen Pseudomonas mendocina ?
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?
additional information experiments with p-deuterotoluene lead to the isolation of p-cresol which retains 68% of the deuterium initially present in the parent molecule. When incubated with toluene in the presence of 1802, the oxygen in p-cresol is derived from molecular oxygen Pseudomonas mendocina KR1 ?
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toluene + NADH + H+ + O2
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Pseudomonas mendocina p-cresol + NAD+ + H2O
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toluene + NADH + H+ + O2
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Pseudomonas mendocina KR1 p-cresol + NAD+ + H2O
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?