Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas mendocina |
Crystallization (Comment) | Organism |
---|---|
comparison of X-ray crystal structures of resting and reduced mutant T201A in complex with effector T4moD with structures of wild-type reveals changes in the positions of several key active site residues | Pseudomonas mendocina |
Protein Variants | Comment | Organism |
---|---|---|
T201A | mutation has no impact on steady-state catalysis or coupling. Mutant T201A gives stoichometric release of H2O2 during reaction in the absence of substrate and has a faster first-order rate constant for product formation than wild-type | Pseudomonas mendocina |
T201G | mutation has no impact on steady-state catalysis or coupling | Pseudomonas mendocina |
T201S | mutation has no impact on steady-state catalysis or coupling | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q00456 | subunit TmoA | - |
Pseudomonas mendocina KR1 | Q00456 | subunit TmoA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anisole + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-methoxyphenol + NAD+ + H2O | 87% efficiency | ? | |
anisole + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-methoxyphenol + NAD+ + H2O | 87% efficiency | ? | |
benzene + NADH + H+ + O2 | - |
Pseudomonas mendocina | phenol + NAD+ + H2O | 98% efficiency | ? | |
benzene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | phenol + NAD+ + H2O | 98% efficiency | ? | |
chlorobenzene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-chlorophenol + NAD+ + H2O | 39% efficiency | ? | |
chlorobenzene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-chlorophenol + NAD+ + H2O | 39% efficiency | ? | |
nitrobenzene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-nitrophenol + NAD+ + H2O | 12% efficiency | ? | |
nitrobenzene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-nitrophenol + NAD+ + H2O | 12% efficiency | ? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-methylphenol + NAD+ + H2O | 194% efficiency | ? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-methylphenol + NAD+ + H2O | 194% efficiency | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
nitrobenzene | wild-type, pH 7.5, 4°C | Pseudomonas mendocina | |
0.034 | - |
nitrobenzene | mutant T201A, pH 7.5, 4°C | Pseudomonas mendocina | |
0.075 | - |
chlorobenzene | wild-type, pH 7.5, 4°C | Pseudomonas mendocina | |
0.087 | - |
chlorobenzene | mutant T201A, pH 7.5, 4°C | Pseudomonas mendocina | |
0.1 | - |
Benzene | wild-type, pH 7.5, 4°C | Pseudomonas mendocina | |
0.16 | - |
anisole | wild-type, pH 7.5, 4°C | Pseudomonas mendocina | |
0.21 | - |
Toluene | wild-type, pH 7.5, 4°C | Pseudomonas mendocina | |
0.23 | - |
Toluene | mutant T201A, pH 7.5, 4°C | Pseudomonas mendocina | |
0.23 | - |
anisole | mutant T201A, pH 7.5, 4°C | Pseudomonas mendocina | |
0.31 | - |
Benzene | mutant T201A, pH 7.5, 4°C | Pseudomonas mendocina |
General Information | Comment | Organism |
---|---|---|
physiological function | during reactions in the absence of substrate, wild-type toluene 4-monooxygenase hydroxylase T4moH slowly consumes O2 but only a negligible amount of H2O2 is released | Pseudomonas mendocina |