Application | Comment | Organism |
---|---|---|
synthesis | conversion of benzene to phenol and naphthalene to 2-naphthol in a two-phase system using whole cells expressing wild-type toluene 4-monooxygenase and the alpha subunit variant TmoA I100A. With mutant I100A, the solubility of naphthalene is enhanced and the toxicity of the naphthols is prevented by the use of a water/dioctyl phthalate (80:20, vol%) system More than 99%2-naphthol is extracted to the dioctyl phthalate phase, dihydroxynaphthalene formation is prevented, 92% 2-naphthol is formed, and 12% naphthalene is converted. Using 50 vol% dioctyl phthalate, and wild-type T4MO, a 51% conversion of benzene is obtained and phenol is produced at a purity of 97% | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q00456 | - |
- |
Pseudomonas mendocina KR1 | Q00456 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene + NADH + H+ + O2 | - |
Pseudomonas mendocina | phenol + NAD+ + H2O | - |
? | |
benzene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | phenol + NAD+ + H2O | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 2-naphthol + NAD+ + H2O | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 2-naphthol + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TmoA | - |
Pseudomonas mendocina |
toluene-4-monooxygenase system protein A | - |
Pseudomonas mendocina |