Cloned (Comment) | Organism |
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expression in Streptomyces lividans and Escherichia coli | Streptomyces rimosus subsp. rimosus |
Crystallization (Comment) | Organism |
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structure of OxyS in complex with oxidized flavin to 2.6 A resolution. The monomeric OxyS is comprised of three structural domains, including the FAD-binding domain (residues 1-175 and 271-389), the middle domain (residues 176-270), and the C-terminal thioredoxin-like domain (residues 390?503). The tetracycline substrate is anchored in a narrow hydrophobic cleft at the interface between the FAD-binding and the middle domains | Streptomyces rimosus subsp. rimosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rimosus subsp. rimosus | L8EUQ6 | bifunctional enzyme, catalyzes the stereospecific hydroxylation of anhydrotetracycline at C-6 (EC 1.14.13.38) and performs an additional hydroxylation at C-5, producing 5a,11a-dehydrooxytetracycline, reaction of EC 1.14.13.234 | - |
Streptomyces rimosus subsp. rimosus ATCC 10970 | L8EUQ6 | bifunctional enzyme, catalyzes the stereospecific hydroxylation of anhydrotetracycline at C-6 (EC 1.14.13.38) and performs an additional hydroxylation at C-5, producing 5a,11a-dehydrooxytetracycline, reaction of EC 1.14.13.234 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5a,11a-dehydrotetracycline + NADPH + H+ + O2 | - |
Streptomyces rimosus subsp. rimosus | 5a,11a-dehydrooxytetracycline + NADP+ + H2O | - |
? | |
5a,11a-dehydrotetracycline + NADPH + H+ + O2 | - |
Streptomyces rimosus subsp. rimosus ATCC 10970 | 5a,11a-dehydrooxytetracycline + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oxyS | - |
Streptomyces rimosus subsp. rimosus |
General Information | Comment | Organism |
---|---|---|
physiological function | OxyS catalyzes two sequential hydroxylations at C6 and C5 positions of anhydrotetracycline with opposite stereochemistry. OxyS and reductase OxyR are sufficient to produce the mature tetracycline scaffold, i.e. the complete conversion of anhydrotetracycline to oxytetracycline and tetracycline. OxyS catalyzes the stereospecific hydroxylation of anhydrotetracycline at C-6 (reaction of EC 1.14.13.38). If the released product is captured by EC 1.3.98.4, 5a,11a-dehydrotetracycline dehydrogenase (OxyR), it is reduced to tetracycline. However, if the released product is recaptured by OxyS, it performs an additional hydroxylation at C-5, producing 5a,11a-dehydrooxytetracycline, which, following the action of OxyR, becomes oxytetracycline | Streptomyces rimosus subsp. rimosus |