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Literature summary for 1.14.13.231 extracted from

  • Moore, I.F.; Hughes, D.W.; Wright, G.D.
    Tigecycline is modified by the flavin-dependent monooxygenase TetX (2005), Biochemistry, 44, 11829-11835.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 tigecycline pH 8.5, temperature not specified in the publication Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tigecycline + NADPH + H+ + O2
-
 Bacteroides fragilis 11a-hydroxytigecycline + NADP+ + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
 tigecycline pH 8.5, temperature not specified in the publication Bacteroides fragilis

General Information

General Information Comment Organism
physiological function tigecycline is a substrate for TetX and bacterial strains containing the TetX gene are resistant to tigecycline due to the modification of tigecycline by TetX to form 11a-hydroxytigecycline, which has a weakened ability to inhibit protein translation compared with tigecycline. 11a-Hydroxytigecycline forms a weaker complex with magnesium than tigecycline, magnesium coordination is critical for binding tetracycline to the ribosome Bacteroides fragilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.3
-
 tigecycline pH 8.5, temperature not specified in the publication Bacteroides fragilis