BRENDA - Enzyme Database show
show all sequences of 1.14.13.230

Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly Pseudomonas butanovora

Cooley, R.B.; Dubbels, B.L.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.; Microbiology 155, 2086-2096 (2009)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00024
-
butane
pH 7.2, 25°C
Thauera butanivorans
0.00034
-
pentane
pH 7.2, 25°C
Thauera butanivorans
0.00094
-
propane
pH 7.2, 25°C
Thauera butanivorans
0.0022
-
ethane
pH 7.2, 25°C
Thauera butanivorans
1.1
-
methane
pH 7.2, 25°C
Thauera butanivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thauera butanivorans
Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
Thauera butanivorans ATCC 43655
Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butane + NADH + H+ + O2
-
739010
Thauera butanivorans
butan-1-ol + NAD+ + H2O
-
-
-
?
butane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
butan-1-ol + NAD+ + H2O
-
-
-
?
butane + NADH + H+ + O2
-
739010
Thauera butanivorans
butan-2-ol + NAD+ + H2O
-
-
-
?
butane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
butan-2-ol + NAD+ + H2O
-
-
-
?
ethane + NADH + H+ + O2
-
739010
Thauera butanivorans
ethanol + NAD+ + H2O
-
-
-
?
ethane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
ethanol + NAD+ + H2O
-
-
-
?
methane + NADH + H+ + O2
-
739010
Thauera butanivorans
methanol + NAD+ + H2O
-
-
-
?
methane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
methanol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans
pentan-1-ol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
pentan-1-ol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans
pentan-2-ol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans
pentan-3-ol + NAD+ + H2O
-
-
-
?
propane + NADH + H+ + O2
-
739010
Thauera butanivorans
propan-1-ol + NAD+ + H2O
-
-
-
?
propane + NADH + H+ + O2
-
739010
Thauera butanivorans
propan-2-ol + NAD+ + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.008
-
pentane
product pentan-3-ol, pH 7.2, 25°C
Thauera butanivorans
0.039
-
pentane
product pentan-2-ol, pH 7.2, 25°C
Thauera butanivorans
0.12
-
butane
product butan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.34
-
pentane
product pentan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.48
-
butane
product butan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.55
-
propane
product propan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.76
-
ethane
pH 7.2, 25°C
Thauera butanivorans
0.98
-
propane
product propan-2-ol, pH 7.2, 25°C
Thauera butanivorans
1.3
-
methane
pH 7.2, 25°C
Thauera butanivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00024
-
butane
pH 7.2, 25°C
Thauera butanivorans
0.00034
-
pentane
pH 7.2, 25°C
Thauera butanivorans
0.00094
-
propane
pH 7.2, 25°C
Thauera butanivorans
0.0022
-
ethane
pH 7.2, 25°C
Thauera butanivorans
1.1
-
methane
pH 7.2, 25°C
Thauera butanivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butane + NADH + H+ + O2
-
739010
Thauera butanivorans
butan-1-ol + NAD+ + H2O
-
-
-
?
butane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
butan-1-ol + NAD+ + H2O
-
-
-
?
butane + NADH + H+ + O2
-
739010
Thauera butanivorans
butan-2-ol + NAD+ + H2O
-
-
-
?
butane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
butan-2-ol + NAD+ + H2O
-
-
-
?
ethane + NADH + H+ + O2
-
739010
Thauera butanivorans
ethanol + NAD+ + H2O
-
-
-
?
ethane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
ethanol + NAD+ + H2O
-
-
-
?
methane + NADH + H+ + O2
-
739010
Thauera butanivorans
methanol + NAD+ + H2O
-
-
-
?
methane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
methanol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans
pentan-1-ol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans ATCC 43655
pentan-1-ol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans
pentan-2-ol + NAD+ + H2O
-
-
-
?
pentane + NADH + H+ + O2
-
739010
Thauera butanivorans
pentan-3-ol + NAD+ + H2O
-
-
-
?
propane + NADH + H+ + O2
-
739010
Thauera butanivorans
propan-1-ol + NAD+ + H2O
-
-
-
?
propane + NADH + H+ + O2
-
739010
Thauera butanivorans
propan-2-ol + NAD+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.008
-
pentane
product pentan-3-ol, pH 7.2, 25°C
Thauera butanivorans
0.039
-
pentane
product pentan-2-ol, pH 7.2, 25°C
Thauera butanivorans
0.12
-
butane
product butan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.34
-
pentane
product pentan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.48
-
butane
product butan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.55
-
propane
product propan-1-ol, pH 7.2, 25°C
Thauera butanivorans
0.76
-
ethane
pH 7.2, 25°C
Thauera butanivorans
0.98
-
propane
product propan-2-ol, pH 7.2, 25°C
Thauera butanivorans
1.3
-
methane
pH 7.2, 25°C
Thauera butanivorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2
-
methane
pH 7.2, 25°C
Thauera butanivorans
350
-
ethane
pH 7.2, 25°C
Thauera butanivorans
690
-
propane
overall reaction, pH 7.2, 25°C
Thauera butanivorans
1200
-
pentane
overall reaction, pH 7.2, 25°C
Thauera butanivorans
2500
-
butane
overall reaction, pH 7.2, 25°C
Thauera butanivorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2
-
methane
pH 7.2, 25°C
Thauera butanivorans
350
-
ethane
pH 7.2, 25°C
Thauera butanivorans
690
-
propane
overall reaction, pH 7.2, 25°C
Thauera butanivorans
1200
-
pentane
overall reaction, pH 7.2, 25°C
Thauera butanivorans
2500
-
butane
overall reaction, pH 7.2, 25°C
Thauera butanivorans
Other publictions for EC 1.14.13.230
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739010
Cooley
Kinetic characterization of th ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
155
2086-2096
2009
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-
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-
-
-
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5
-
-
-
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5
-
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-
-
-
-
-
14
-
-
-
-
9
-
-
-
-
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-
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-
-
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5
-
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-
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-
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-
14
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9
-
-
-
-
-
-
-
-
5
5
739007
Dubbels
Butane monooxygenase of Pseudo ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
1808-1816
2007
-
-
-
-
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-
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-
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-
5
-
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-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739008
Doughty
Propionate inactivation of but ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
3722-3729
2007
-
-
-
-
5
-
2
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738517
Halsey
Site-directed amino acid subst ...
Thauera butanivorans
J. Bacteriol.
188
4962-4969
2006
-
-
-
-
9
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
673717
Sayavedra-Soto
Product and product-independen ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
FEMS Microbiol. Lett.
250
111-116
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
739005
Sluis
Molecular analysis of the solu ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
148
3617-3629
2002
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-