BRENDA - Enzyme Database show
show all sequences of 1.14.13.230

Propionate inactivation of butane monooxygenase activity in Pseudomonas butanovora: biochemical and physiological implications

Doughty, D.M.; Halsey, K.H.; Vieville, C.J.; Sayavedra-Soto, L.A.; Arp, D.J.; Bottomley, P.J.; Microbiology 153, 3722-3729 (2007)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
F321Y
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 61% residual activity
Thauera butanivorans
G113N
mutation in the alpha-subunit of hydrolase component, loss of inhibition by propanoate
Thauera butanivorans
L279F
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 56% residual activity
Thauera butanivorans
Q320K
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 28% residual activity
Thauera butanivorans
T148C
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 15% residual activity
Thauera butanivorans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Butanoate
incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity
Thauera butanivorans
propanoate
10 mM, 41% residual activity. Incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity. Propanoate-dependent inactivation involves interaction with the catalytic site. Butane monooxygenase is protected from propanoate-dependent inactivation by the presence of its natural substrate, butane
Thauera butanivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thauera butanivorans
Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
Thauera butanivorans ATCC 43655
Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F321Y
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 61% residual activity
Thauera butanivorans
G113N
mutation in the alpha-subunit of hydrolase component, loss of inhibition by propanoate
Thauera butanivorans
L279F
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 56% residual activity
Thauera butanivorans
Q320K
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 28% residual activity
Thauera butanivorans
T148C
mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 15% residual activity
Thauera butanivorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Butanoate
incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity
Thauera butanivorans
propanoate
10 mM, 41% residual activity. Incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity. Propanoate-dependent inactivation involves interaction with the catalytic site. Butane monooxygenase is protected from propanoate-dependent inactivation by the presence of its natural substrate, butane
Thauera butanivorans
Other publictions for EC 1.14.13.230
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739010
Cooley
Kinetic characterization of th ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
155
2086-2096
2009
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5
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5
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14
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9
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5
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14
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9
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5
5
739007
Dubbels
Butane monooxygenase of Pseudo ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
1808-1816
2007
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5
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12
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12
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739008
Doughty
Propionate inactivation of but ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
3722-3729
2007
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5
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2
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5
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5
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2
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738517
Halsey
Site-directed amino acid subst ...
Thauera butanivorans
J. Bacteriol.
188
4962-4969
2006
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9
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1
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1
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9
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1
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1
1
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673717
Sayavedra-Soto
Product and product-independen ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
FEMS Microbiol. Lett.
250
111-116
2005
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6
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2
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2
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1
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1
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739005
Sluis
Molecular analysis of the solu ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
148
3617-3629
2002
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2
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5
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2
1
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1
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1
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2
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1
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1
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