BRENDA - Enzyme Database show
show all sequences of 1.14.13.230

Butane monooxygenase of Pseudomonas butanovora: purification and biochemical characterization of a terminal-alkane hydroxylating diiron monooxygenase

Dubbels, B.L.; Sayavedra-Soto, L.A.; Arp, D.J.; Microbiology 153, 1808-1816 (2007)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thauera butanivorans
Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
Thauera butanivorans ATCC 43655
Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butane + NADH + H+ + O2
-
739007
Thauera butanivorans
butan-1-ol + NAD+ + H2O
about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB
-
-
?
butane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
butan-1-ol + NAD+ + H2O
about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB
-
-
?
hexane + NADH + H+ + O2
-
739007
Thauera butanivorans
hexan-1-ol + NAD+ + H2O
about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively
-
-
?
hexane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
hexan-1-ol + NAD+ + H2O
about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively
-
-
?
isobutane + NADH + H+ + O2
-
739007
Thauera butanivorans
2-methyl-1-propanol + NAD+ + H2O
about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively
-
-
?
isobutane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
2-methyl-1-propanol + NAD+ + H2O
about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively
-
-
?
isopentane + NADH + H+ + O2
-
739007
Thauera butanivorans
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively
-
-
?
isopentane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively
-
-
?
additional information
the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene
739007
Thauera butanivorans
?
-
-
-
-
additional information
the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene
739007
Thauera butanivorans ATCC 43655
?
-
-
-
-
pentane + NADH + H+ + O2
-
739007
Thauera butanivorans
pentan-1-ol + NAD+ + H2O
about 80% pentan-1-ol plus 13% pentan-2-ol and 7% pentan-3-ol in absence, and 88% pentan-1-ol plus 11% pentan-2-ol and 1% pentan-3-ol in presence of regulatory subunit BmoB, respectively
-
-
?
propane + NADH + H+ + O2
-
739007
Thauera butanivorans
propan-1-ol + NAD+ + H2O
about 82% propan-1-ol plus 18% propan-2-ol, in presence and absence of regulatory subunit BmoB
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butane + NADH + H+ + O2
-
739007
Thauera butanivorans
butan-1-ol + NAD+ + H2O
about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB
-
-
?
butane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
butan-1-ol + NAD+ + H2O
about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB
-
-
?
hexane + NADH + H+ + O2
-
739007
Thauera butanivorans
hexan-1-ol + NAD+ + H2O
about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively
-
-
?
hexane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
hexan-1-ol + NAD+ + H2O
about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively
-
-
?
isobutane + NADH + H+ + O2
-
739007
Thauera butanivorans
2-methyl-1-propanol + NAD+ + H2O
about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively
-
-
?
isobutane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
2-methyl-1-propanol + NAD+ + H2O
about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively
-
-
?
isopentane + NADH + H+ + O2
-
739007
Thauera butanivorans
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively
-
-
?
isopentane + NADH + H+ + O2
-
739007
Thauera butanivorans ATCC 43655
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively
-
-
?
additional information
the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene
739007
Thauera butanivorans
?
-
-
-
-
additional information
the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene
739007
Thauera butanivorans ATCC 43655
?
-
-
-
-
pentane + NADH + H+ + O2
-
739007
Thauera butanivorans
pentan-1-ol + NAD+ + H2O
about 80% pentan-1-ol plus 13% pentan-2-ol and 7% pentan-3-ol in absence, and 88% pentan-1-ol plus 11% pentan-2-ol and 1% pentan-3-ol in presence of regulatory subunit BmoB, respectively
-
-
?
propane + NADH + H+ + O2
-
739007
Thauera butanivorans
propan-1-ol + NAD+ + H2O
about 82% propan-1-ol plus 18% propan-2-ol, in presence and absence of regulatory subunit BmoB
-
-
?
Other publictions for EC 1.14.13.230
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739010
Cooley
Kinetic characterization of th ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
155
2086-2096
2009
-
-
-
-
-
-
-
5
-
-
-
-
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
9
-
-
-
-
-
-
-
-
5
5
739007
Dubbels
Butane monooxygenase of Pseudo ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
1808-1816
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739008
Doughty
Propionate inactivation of but ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
3722-3729
2007
-
-
-
-
5
-
2
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738517
Halsey
Site-directed amino acid subst ...
Thauera butanivorans
J. Bacteriol.
188
4962-4969
2006
-
-
-
-
9
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
673717
Sayavedra-Soto
Product and product-independen ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
FEMS Microbiol. Lett.
250
111-116
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
739005
Sluis
Molecular analysis of the solu ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
148
3617-3629
2002
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-