BRENDA - Enzyme Database show
show all sequences of 1.14.13.230

Site-directed amino acid substitutions in the hydroxylase alpha subunit of butane monooxygenase from Pseudomonas butanovora: Implications for substrates knocking at the gate

Halsey, K.H.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.; J. Bacteriol. 188, 4962-4969 (2006)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
F321Y
mutation in subunit BmoX towards soluble methane monooxygenase, no formation of butan-2-ol
Thauera butanivorans
G113N
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 11.1
Thauera butanivorans
L279F
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.22
Thauera butanivorans
L287F
mutation in subunit BmoX, loss of activity
Thauera butanivorans
P179K
mutation in subunit BmoX, loss of activity
Thauera butanivorans
Q319G
mutation in subunit BmoX, loss of activity
Thauera butanivorans
Q320K
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.20
Thauera butanivorans
T148C
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.15
Thauera butanivorans
V181M
mutation in subunit BmoX, loss of activity
Thauera butanivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thauera butanivorans
Q8KQF0 and Q8KQE9 and Q8KQE7
Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively
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Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butane + NADH + H+ + O2
-
738517
Thauera butanivorans
butan-1-ol + NAD+ + H2O
formation of about 3.6% of butan-2-ol
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F321Y
mutation in subunit BmoX towards soluble methane monooxygenase, no formation of butan-2-ol
Thauera butanivorans
G113N
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 11.1
Thauera butanivorans
L279F
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.22
Thauera butanivorans
L287F
mutation in subunit BmoX, loss of activity
Thauera butanivorans
P179K
mutation in subunit BmoX, loss of activity
Thauera butanivorans
Q319G
mutation in subunit BmoX, loss of activity
Thauera butanivorans
Q320K
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.20
Thauera butanivorans
T148C
mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.15
Thauera butanivorans
V181M
mutation in subunit BmoX, loss of activity
Thauera butanivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butane + NADH + H+ + O2
-
738517
Thauera butanivorans
butan-1-ol + NAD+ + H2O
formation of about 3.6% of butan-2-ol
-
-
?
General Information
General Information
Commentary
Organism
physiological function
except for C1, strain BCP1 cells grow on the tested short-chain n-alkanes and show the lowest generation times with C3-C5 n-alkanes (2.7–2.8 h)
Thauera butanivorans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
except for C1, strain BCP1 cells grow on the tested short-chain n-alkanes and show the lowest generation times with C3-C5 n-alkanes (2.7–2.8 h)
Thauera butanivorans
Other publictions for EC 1.14.13.230
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739010
Cooley
Kinetic characterization of th ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
155
2086-2096
2009
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5
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5
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14
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9
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5
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14
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9
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5
5
739007
Dubbels
Butane monooxygenase of Pseudo ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
1808-1816
2007
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5
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12
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12
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739008
Doughty
Propionate inactivation of but ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
153
3722-3729
2007
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5
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2
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5
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5
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2
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738517
Halsey
Site-directed amino acid subst ...
Thauera butanivorans
J. Bacteriol.
188
4962-4969
2006
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9
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1
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1
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9
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1
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1
1
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673717
Sayavedra-Soto
Product and product-independen ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
FEMS Microbiol. Lett.
250
111-116
2005
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6
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2
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2
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1
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1
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739005
Sluis
Molecular analysis of the solu ...
Thauera butanivorans, Thauera butanivorans ATCC 43655
Microbiology
148
3617-3629
2002
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2
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5
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2
1
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1
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1
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2
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2
1
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1
1
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