Protein Variants | Comment | Organism |
---|---|---|
F321Y | mutation in subunit BmoX towards soluble methane monooxygenase, no formation of butan-2-ol | Thauera butanivorans |
G113N | mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 11.1 | Thauera butanivorans |
L279F | mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.22 | Thauera butanivorans |
L287F | mutation in subunit BmoX, loss of activity | Thauera butanivorans |
P179K | mutation in subunit BmoX, loss of activity | Thauera butanivorans |
Q319G | mutation in subunit BmoX, loss of activity | Thauera butanivorans |
Q320K | mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.20 | Thauera butanivorans |
T148C | mutation in subunit BmoX towards soluble methane monooxygenase, ratio of 2-butanol to 1-butanol accumulation increases from 0.04 for wild-type to 0.15 | Thauera butanivorans |
V181M | mutation in subunit BmoX, loss of activity | Thauera butanivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera butanivorans | Q8KQF0 and Q8KQE9 and Q8KQE7 | Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butane + NADH + H+ + O2 | - |
Thauera butanivorans | butan-1-ol + NAD+ + H2O | formation of about 3.6% of butan-2-ol | ? |
Synonyms | Comment | Organism |
---|---|---|
BmoX | - |
Thauera butanivorans |
BmoY | - |
Thauera butanivorans |
BmoZ | - |
Thauera butanivorans |
General Information | Comment | Organism |
---|---|---|
physiological function | except for C1, strain BCP1 cells grow on the tested short-chain n-alkanes and show the lowest generation times with C3-C5 n-alkanes (2.72.8 h) | Thauera butanivorans |