Literature summary for 1.14.13.23 extracted from

Hiromoto, T.; Matsue, H.; Yoshida, M.; Tanaka, T.; Higashibata, H.; Hosokawa, K.; Yamaguchi, H.; Fujiwara, S.
Characterization of MobR, the 3-hydroxybenzoate-responsive transcriptional regulator for the 3-hydroxybenzoate hydroxylase gene of Comamonas testosteroni KH122-3s (2006), J. Mol. Biol., 364, 863-877.

Search for more literature for 1.14.13.23

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme is specifically induced by 3-hydroxybenzoate, no activation by 4-hydroxybenzoate	Comamonas testosteroni

Cloned(Commentary)

Cloned (Comment)	Organism
gene mobA, promoter study, primer extension method, transription regulation/repression by MobR, genetic organization, overview, overexpression of mobA and of a mobA-mobR fusion construct in Escherichia coli	Comamonas testosteroni

Inhibitors

Inhibitors	Comment	Organism	Structure
MobR	a 42 kDa dimeric transcriptional regulator of the MarR family, encoded by an open reading frame mobR in the upstream region of mobA, binds to the target DNA and negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate, binding kinetics, overview	Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-hydroxybenzoate + NADH + O2	Comamonas testosteroni	initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate	3,4-dihydroxybenzoate + NAD+ + H2O	-	?
3-hydroxybenzoate + NADH + O2	Comamonas testosteroni KH122-3s	initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate	3,4-dihydroxybenzoate + NAD+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Comamonas testosteroni	-	-	-
Comamonas testosteroni KH122-3s	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the aerobic soil bacterium that utilizes 3-hydroxybenzoate as a sole carbon and energy source	Comamonas testosteroni	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.002	-	uninduced wild-type strain	Comamonas testosteroni
0.055	-	wild-type strain induced by 3-hydroxybenzoate	Comamonas testosteroni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxybenzoate + NADH + O2	-	Comamonas testosteroni	3,4-dihydroxybenzoate + NAD+ + H2O	-	?
3-hydroxybenzoate + NADH + O2	initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate	Comamonas testosteroni	3,4-dihydroxybenzoate + NAD+ + H2O	-	?
3-hydroxybenzoate + NADH + O2	-	Comamonas testosteroni KH122-3s	3,4-dihydroxybenzoate + NAD+ + H2O	-	?
3-hydroxybenzoate + NADH + O2	initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate	Comamonas testosteroni KH122-3s	3,4-dihydroxybenzoate + NAD+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
3-hydroxybenzoate hydroxylase	-	Comamonas testosteroni
MobA	-	Comamonas testosteroni

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
24	-	assay at	Comamonas testosteroni

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Comamonas testosteroni
NADPH	-	Comamonas testosteroni

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Release 2023.1 (January 2023)