KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | + 2-fluoro-5-hydroxybenzoate | Comamonas testosteroni | |
0.03 | - |
3-hydroxybenzoate | cosubstrate NADPH | Comamonas testosteroni | |
0.04 | - |
3,5-Dihydroxybenzoate | cosubstrate NADPH | Comamonas testosteroni | |
0.05 | - |
NADPH | cosubstrates 3-hydroxyanthranilate or 3,5-dihydroxybenzoate | Comamonas testosteroni | |
0.06 | - |
2,3-Dihydroxybenzoate | cosubstrate NADPH | Comamonas testosteroni | |
0.07 | - |
NADPH | cosubstrates 3-hydroxybenzoate or 2,3-dihydroxybenzoate | Comamonas testosteroni | |
0.12 | - |
4-Fluoro-3-hydroxybenzoate | cosubstrate NADPH | Comamonas testosteroni | |
0.14 | - |
NADPH | + 2,5-dihydroxybenzoate | Comamonas testosteroni | |
0.15 | - |
NADPH | + 4-fluoro-3-hydroxybenzoate | Comamonas testosteroni | |
0.5 | - |
2,5-Dihydroxybenzoate | cosubstrate NADPH | Comamonas testosteroni | |
3 | - |
NADH | cosubstrate 3-hydroxybenzoate | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
sedimentation equilibrium centrifugation | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | Comamonas testosteroni | - |
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.8 | - |
- |
Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
2,5-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
2-fluoro-5-hydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
3,5-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
3-hydroxyanthranilate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
3-hydroxybenzoate + NADH + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
4-fluoro-3-hydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Comamonas testosteroni | |
NADH | poor substitute for NADPH | Comamonas testosteroni | |
NADPH | - |
Comamonas testosteroni |