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Literature summary for 1.14.13.23 extracted from

  • Chen, R.; Oki, H.; Scott, R.P.; Yamaguchi, H.; Kusunoki, M.; Matsuura, Y.; Chaen, H.; Tsugita, A.; Hosokawa, K.
    Crystallization and further characterization of meta-hydroxybenzoate 4-hydroxylase from Comamonas testosteroni (1998), Res. Commun. Biochem. Cell Mol. Biol., 2, 253-274.
No PubMed abstract available
Search for more literature for 1.14.13.23

Crystallization (Commentary)

Crystallization (Comment) Organism
1.4-1.6 M ammonium sulfate, 4-8% dioxane, pH 6.5 Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxy-3-iodomethylbenzoate inhibition not reversed in presence of dithiotreitol Comamonas testosteroni
iodoacetamide inhibition reversed in presence of dithiotreitol Comamonas testosteroni
N-iodosuccinimide inhibition reversed in presence of dithiotreitol Comamonas testosteroni
o-Iodosobenzoate inhibition reversed in presence of dithiotreitol Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 x * 71000, SDS-PAGE Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 Comamonas testosteroni
-
 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.03
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Comamonas testosteroni 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
gentisate + NADPH + O2
-
 Comamonas testosteroni ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, SDS-PAGE Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 in potassium phosphate buffer Comamonas testosteroni
7.3
-
 in Tris-HCl buffer Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Comamonas testosteroni
NADPH specific requirement for NADPH Comamonas testosteroni