Crystallization (Comment) | Organism |
---|---|
1.4-1.6 M ammonium sulfate, 4-8% dioxane, pH 6.5 | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy-3-iodomethylbenzoate | inhibition not reversed in presence of dithiotreitol | Comamonas testosteroni | |
iodoacetamide | inhibition reversed in presence of dithiotreitol | Comamonas testosteroni | |
N-iodosuccinimide | inhibition reversed in presence of dithiotreitol | Comamonas testosteroni | |
o-Iodosobenzoate | inhibition reversed in presence of dithiotreitol | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
x * 71000, SDS-PAGE | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | Comamonas testosteroni | - |
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.03 | - |
- |
Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
gentisate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, SDS-PAGE | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
in potassium phosphate buffer | Comamonas testosteroni |
7.3 | - |
in Tris-HCl buffer | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Comamonas testosteroni | |
NADPH | specific requirement for NADPH | Comamonas testosteroni |