Literature summary for 1.14.13.22 extracted from

Schmidt, S.; Doerr, M.; Bornscheuer, U.T.
Library Growth and Protein Expression Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli Using MTP Shakers (2018), Methods Mol. Biol., 1685, 145-156 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. A protocol is developed for an optimal and reproducible protein expression in microtiter plates showcased for the expression of the cyclohexanone monooxygenase (CMO) from Acinetobacter sp. NCIMB 9871. By emerging this protocol, several parameters concerning the expression medium, the cultivation temperatures, shaking conditions as well as time and induction periods for CHMO are investigated. A microtiter plate shaker with humidity and temperature control (Cytomat) (integrated in a robotic platform) is employed to obtain an even growth and expression over the plates. An optimized protocol provides a comprehensive overview of the key factors influencing a reproducible protein expression	Acinetobacter johnsonii

Cloned (Comment)

Organism

expression in Escherichia coli. A protocol is developed for an optimal and reproducible protein expression in microtiter plates showcased for the expression of the cyclohexanone monooxygenase (CMO) from Acinetobacter sp. NCIMB 9871. By emerging this protocol, several parameters concerning the expression medium, the cultivation temperatures, shaking conditions as well as time and induction periods for CHMO are investigated. A microtiter plate shaker with humidity and temperature control (Cytomat) (integrated in a robotic platform) is employed to obtain an even growth and expression over the plates. An optimized protocol provides a comprehensive overview of the key factors influencing a reproducible protein expression

Acinetobacter johnsonii

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter johnsonii	Q9R2F5	-	-

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Release 2023.1 (January 2023)