Application | Comment | Organism |
---|---|---|
synthesis | a fusion protein that can convert cyclohexanol to epsilon-caprolactone in vitro is created. The alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct is expressed in Escherichia coli cells. By circumventing substrate and product inhibition, a more than 99% conversion of 200 mM cyclohexanol can be achieved in 24 h, with more than 13000 turnovers per fusion enzyme molecule | Thermocrispum municipale |
Cloned (Comment) | Organism |
---|---|
alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct expressed in Escherichia coli cells | Thermocrispum municipale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermocrispum municipale | A0A1L1QK39 | - |
- |
Purification (Comment) | Organism |
---|---|
alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct expressed in Escherichia coli cells | Thermocrispum municipale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + H+ + O2 | - |
Thermocrispum municipale | hexano-6-lactone + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHMO | - |
Thermocrispum municipale |