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Literature summary for 1.14.13.22 extracted from

  • Aalbers, F.S.; Fraaije, M.W.
    Coupled reactions by coupled enzymes alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions (2017), Appl. Microbiol. Biotechnol., 101, 7557-7565 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis a fusion protein that can convert cyclohexanol to epsilon-caprolactone in vitro is created. The alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct is expressed in Escherichia coli cells. By circumventing substrate and product inhibition, a more than 99% conversion of 200 mM cyclohexanol can be achieved in 24 h, with more than 13000 turnovers per fusion enzyme molecule Thermocrispum municipale

Cloned(Commentary)

Cloned (Comment) Organism
alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct expressed in Escherichia coli cells Thermocrispum municipale

Organism

Organism UniProt Comment Textmining
Thermocrispum municipale A0A1L1QK39
-
-

Purification (Commentary)

Purification (Comment) Organism
alcohol dehydrogenase(Mesotoga infera)/cyclohexanone monooxygenase(Thermocrispum municipale) fusion construct expressed in Escherichia coli cells Thermocrispum municipale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexanone + NADPH + H+ + O2
-
Thermocrispum municipale hexano-6-lactone + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
CHMO
-
Thermocrispum municipale