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Literature summary for 1.14.13.22 extracted from

  • Mihovilovic, M.D.; Rudroff, F.; Winninger, A.; Schneider, T.; Schulz, F.; Reetz, M.T.
    Microbial Baeyer-Villiger oxidation: stereopreference and substrate acceptance of cyclohexanone monooxygenase mutants prepared by directed evolution (2006), Org. Lett., 8, 1221-1224.
    View publication on PubMed
Search for more literature for 1.14.13.22

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutants in Escherichia coli Acinetobacter sp.

Protein Variants

Protein Variants Comment Organism
F432I random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
F432S random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
F432Y/K500R random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
K78E/F432S random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
L426P/A541V random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
additional information screening of random mutants of the enzyme against a library of structurally diverse ketones for modifications in the substrate acceptance profile and stereopreference of the enzymatic Baeyer-Villiger biooxidation, improved and/or divergent stereoselectivities are observed for several substrates, overview Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxy-cyclohexanone + NADPH + O2 Acinetobacter sp.
-
 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
4-hydroxy-cyclohexanone + NADPH + O2 Acinetobacter sp. NCIMB 9871
-
 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + O2 Acinetobacter sp.
-
 hexano-6-lactone + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + O2 Acinetobacter sp. NCIMB 9871
-
 hexano-6-lactone + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. NCIMB 9871
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-cyclohexanone + NADPH + O2
-
 Acinetobacter sp. 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
4-hydroxy-cyclohexanone + NADPH + O2 an enantioselective Baeyer-Villiger oxidation Acinetobacter sp. 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
4-hydroxy-cyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIMB 9871 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
4-hydroxy-cyclohexanone + NADPH + O2 an enantioselective Baeyer-Villiger oxidation Acinetobacter sp. NCIMB 9871 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + O2
-
 Acinetobacter sp. hexano-6-lactone + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIMB 9871 hexano-6-lactone + NADP+ + H2O
-
 ?
additional information substrate specificity and enantioselectivity of wild-type and mutant enzymes, overview Acinetobacter sp. ?
-
 ?
additional information substrate specificity and enantioselectivity of wild-type and mutant enzymes, overview Acinetobacter sp. NCIMB 9871 ?
-
 ?

Synonyms

Synonyms Comment Organism
CHMO
-
 Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Acinetobacter sp.