Activating Compound | Comment | Organism | Structure |
---|---|---|---|
7-Chloro-8-demethyl-FAD | reactivates apoenzyme | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-deaza-FAD | competitive | Acinetobacter sp. | |
NADP+ | competitive against NADPH | Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | - |
Acinetobacter sp. | |
0.024 | - |
Thiane | - |
Acinetobacter sp. | |
0.1 | - |
O2 | below | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
native PAGE | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
NCIB 9871 | - |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | reaction mechanism | Acinetobacter sp. | |
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | mechanistic study | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? | |
thiane + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acinetobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Acinetobacter sp. | |
30.3 | - |
cyclohexanone | - |
Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1-deaza-FAD | reactivates apoenzyme | Acinetobacter sp. | |
9-Aza-FAD | reactivates apoenzyme | Acinetobacter sp. | |
FAD | reactivates apoenzyme | Acinetobacter sp. | |
NADPH | - |
Acinetobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADP+ | - |
Acinetobacter sp. | |
0.062 | - |
5-deaza-FAD | - |
Acinetobacter sp. |