Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nocardia farcinica |
Crystallization (Comment) | Organism |
---|---|
in complex with rifampicin, to 1.8 A resolution. RifMO is a class A flavoprotein monooxygenase and is similar in fold and quaternary structure to MtmOIV and OxyS, in the mithramycin and oxytetracycline biosynthetic pathways, respectively. RifMO dimerizes via the FAD-binding domain to form a bell-shaped homodimer in solution with a maximal dimension of 110 A. The rifampicin naphthoquinone blocks access to the FAD N5 atom | Nocardia farcinica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia farcinica | Q5YTV5 | - |
- |
Nocardia farcinica IFM 10152 | Q5YTV5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rifampicin + NAD(P)H + O2 | - |
Nocardia farcinica | 2'-N-hydroxyrifampicin + NAD(P)+ + H2O | - |
? | |
rifampicin + NAD(P)H + O2 | - |
Nocardia farcinica IFM 10152 | 2'-N-hydroxyrifampicin + NAD(P)+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rifampicin monooxygenase | - |
Nocardia farcinica |
RifMO | - |
Nocardia farcinica |
rox | - |
Nocardia farcinica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | NADPH efficiently reduces the FAD only when rifampicin is present, implying that rifampicin binds before NADPH in the catalytic scheme | Nocardia farcinica | |
NADPH | NADPH efficiently reduces the FAD only when rifampicin is present, implying that rifampicin binds before NADPH in the catalytic scheme | Nocardia farcinica |