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Literature summary for 1.14.13.208 extracted from

  • Rather, L.J.; Knapp, B.; Haehnel, W.; Fuchs, G.
    Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation (2010), J. Biol. Chem., 285, 20615-20624.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
BoxC protein
-
Aromatoleum evansii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains iron Aromatoleum evansii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
1 * 54000, BoxB, SDS-PAGE Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
?
benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii KB740
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii
-
-
-
Aromatoleum evansii KB740
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin Superflow column chromatography Aromatoleum evansii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2
-
Aromatoleum evansii 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
?
benzoyl-CoA + NADPH + H+ + O2
-
Aromatoleum evansii KB740 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 54000, BoxB, SDS-PAGE Aromatoleum evansii

Synonyms

Synonyms Comment Organism
benzoyl-CoA oxygenase
-
Aromatoleum evansii
BoxAB
-
Aromatoleum evansii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aromatoleum evansii
Fe-S center
-
Aromatoleum evansii
NADPH
-
Aromatoleum evansii