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Literature summary for 1.14.13.20 extracted from

  • Lu, Y.; Yu, Y.; Zhou, R.; Sun, W.; Dai, C.; Wan, P.; Zhang, L.; Hao, D.; Ren, H.
    Cloning and characterisation of a novel 2,4-dichlorophenol hydroxylase from a metagenomic library derived from polychlorinated biphenyl-contaminated soil (2011), Biotechnol. Lett., 33, 1159-1167.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Soil bacterium

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.1 mM, complete loss of activity Soil bacterium
Hg2+ 0.1 mM, complete loss of activity Soil bacterium
Zn2+ 0.1 mM, complete loss of activity Soil bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
2,4-Dichlorophenol pH 7.5, 25┬░C Soil bacterium
0.006
-
NADPH pH 7.5, 25┬░C Soil bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 mM, 130% of initial activity Soil bacterium
Co2+ 0.1 mM, 110% of initial activity Soil bacterium
Mn2+ 0.1 mM, 121% of initial activity Soil bacterium

Organism

Organism UniProt Comment Textmining
Soil bacterium
-
metagenome constructed from polychlorinated biphenyl-contaminated soil
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dichlorophenol + NADPH + O2 113% of the activity with 2,4-dichlorophenol Soil bacterium 3,4-dichlorocatechol + NADP+ + H2O
-
?
2,4-dichlorophenol + NADPH + H+ + O2
-
Soil bacterium 3,5-dichlorocatechol + NADP+ + H2O
-
?
2,5-dichlorophenol + NADPH + O2 103% of the activity with 2,4-dichlorophenol Soil bacterium 3,6-dichlorocatechol + NADP+ + H2O
-
?
2,5-dichlorophenol + NADPH + O2 178% of the activity with 2,4-dichlorophenol Soil bacterium ? + NADP+ + H2O
-
?
2-bromo-4-chlorophenol + NADPH + O2 82% of the activity with 2,4-dichlorophenol Soil bacterium 3-bromo-5-chlorocatechol + NADP+ + H2O
-
?
2-chlorophenol + NADPH + O2 129% of the activity with 2,4-dichlorophenol Soil bacterium 3-chlorocatechol + NADP+ + H2O
-
?
3-chlorophenol + NADPH + O2 230% of the activity with 2,4-dichlorophenol Soil bacterium 4-chlorocatechol + NADP+ + H2O
-
?
4-bromo-2-chlorophenol + NADPH + O2 86% of the activity with 2,4-dichlorophenol Soil bacterium 5-bromo-3-chlorocatechol + NADP+ + H2O
-
?
4-chloro-2-methylphenol + NADPH + O2 95% of the activity with 2,4-dichlorophenol Soil bacterium 5-chloro-3-methylcatechol + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
TfdB-JLU
-
Soil bacterium

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
-
Soil bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Soil bacterium