Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Soil bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.1 mM, complete loss of activity | Soil bacterium | |
Hg2+ | 0.1 mM, complete loss of activity | Soil bacterium | |
Zn2+ | 0.1 mM, complete loss of activity | Soil bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
2,4-Dichlorophenol | pH 7.5, 25°C | Soil bacterium | |
0.006 | - |
NADPH | pH 7.5, 25°C | Soil bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1 mM, 130% of initial activity | Soil bacterium | |
Co2+ | 0.1 mM, 110% of initial activity | Soil bacterium | |
Mn2+ | 0.1 mM, 121% of initial activity | Soil bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Soil bacterium | - |
metagenome constructed from polychlorinated biphenyl-contaminated soil | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dichlorophenol + NADPH + O2 | 113% of the activity with 2,4-dichlorophenol | Soil bacterium | 3,4-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + H+ + O2 | - |
Soil bacterium | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,5-dichlorophenol + NADPH + O2 | 103% of the activity with 2,4-dichlorophenol | Soil bacterium | 3,6-dichlorocatechol + NADP+ + H2O | - |
? | |
2,5-dichlorophenol + NADPH + O2 | 178% of the activity with 2,4-dichlorophenol | Soil bacterium | ? + NADP+ + H2O | - |
? | |
2-bromo-4-chlorophenol + NADPH + O2 | 82% of the activity with 2,4-dichlorophenol | Soil bacterium | 3-bromo-5-chlorocatechol + NADP+ + H2O | - |
? | |
2-chlorophenol + NADPH + O2 | 129% of the activity with 2,4-dichlorophenol | Soil bacterium | 3-chlorocatechol + NADP+ + H2O | - |
? | |
3-chlorophenol + NADPH + O2 | 230% of the activity with 2,4-dichlorophenol | Soil bacterium | 4-chlorocatechol + NADP+ + H2O | - |
? | |
4-bromo-2-chlorophenol + NADPH + O2 | 86% of the activity with 2,4-dichlorophenol | Soil bacterium | 5-bromo-3-chlorocatechol + NADP+ + H2O | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | 95% of the activity with 2,4-dichlorophenol | Soil bacterium | 5-chloro-3-methylcatechol + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TfdB-JLU | - |
Soil bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Soil bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Soil bacterium |