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Literature summary for 1.14.13.20 extracted from

  • Ledger, T.; Pieper, D.H.; Gonzalez, B.
    Chlorophenol hydroxylases encoded by plasmid pJP4 differentially contribute to chlorophenoxyacetic acid degradation (2006), Appl. Environ. Microbiol., 72, 2783-2792.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.20

Protein Variants

Protein Variants Comment Organism
additional information construction of tfdBI and tfdBII inactivation mutants on pJP4 plasmid, constructed in Escherichia coli strain BW25113 by allelic replacement of each of the genes tfdBI or tfdBII with a kanamycin resistance cassette, tfdBI and tfdBII are required for growth on 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, with tfdBI contributing to a higher extent than tfdBII, phenotype, overview, accumulation of chlorophenol inhibits chlorophenoxyacetate degradation, and inactivation of the tfdB genes enhances the toxic effect of 2,4-dichlorophenoxyacetate on Cupriavidus necator cells Cupriavidus necator
additional information overexpression of tdfA inhibits chlorophenoxyacetate degradation in Burkholderia xenovorans/pJP4 cells Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dichlorophenol + NADPH + O2 Cupriavidus necator
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
2,4-dichlorophenol + NADPH + O2 Paraburkholderia xenovorans
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
4-chloro-2-methylphenol + NADPH + O2 Cupriavidus necator
-
 ?
-
 ?
4-chloro-2-methylphenol + NADPH + O2 Paraburkholderia xenovorans
-
 ?
-
 ?
4-chlorophenol + NADPH + O2 Cupriavidus necator
-
 5-chlorocatechol + NADP+ + H2O
-
 ?
4-chlorophenol + NADPH + O2 Paraburkholderia xenovorans
-
 5-chlorocatechol + NADP+ + H2O
-
 ?
additional information Cupriavidus necator the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview ?
-
 ?
additional information Paraburkholderia xenovorans the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
 strains JMP222 and JMP134 containing the enzyme-encoding plasmid pJP4, genes tfdBI and tfdBII
-
Paraburkholderia xenovorans
-
 containing the enzyme-encoding plasmid pJP4, genes tfdBI and tfdBII
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in vivo degradation activities, overview Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorophenol + NADPH + O2
-
 Cupriavidus necator 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
2,4-dichlorophenol + NADPH + O2
-
 Paraburkholderia xenovorans 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
4-chloro-2-methylphenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?
4-chloro-2-methylphenol + NADPH + O2
-
 Paraburkholderia xenovorans ?
-
 ?
4-chlorophenol + NADPH + O2
-
 Cupriavidus necator 5-chlorocatechol + NADP+ + H2O
-
 ?
4-chlorophenol + NADPH + O2
-
 Paraburkholderia xenovorans 5-chlorocatechol + NADP+ + H2O
-
 ?
additional information the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview Cupriavidus necator ?
-
 ?
additional information the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview Paraburkholderia xenovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
chlorophenol hydroxylase
-
 Cupriavidus necator
chlorophenol hydroxylase
-
 Paraburkholderia xenovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cupriavidus necator
30
-
 assay at Paraburkholderia xenovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Cupriavidus necator
7.6
-
 assay at Paraburkholderia xenovorans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Cupriavidus necator
NADPH
-
 Paraburkholderia xenovorans