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Literature summary for 1.14.13.20 extracted from

  • Beadle, C.A.; Smith, A.R.W.
    The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species (1982), Eur. J. Biochem., 123, 323-332.
    View publication on PubMed

General Stability

General Stability Organism
2,4-dichlorophenol, 0.1 mM, stabilizes during purification Acinetobacter sp.
dithiothreitol, 1 mM, stabilizes during purification Acinetobacter sp.
EDTA, 0.1 mM, stabilizes during purification Acinetobacter sp.
FAD, 0.01 mM, stabilizes during purification Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dichlorophenol above 1 mM Acinetobacter sp.
AgNO3
-
Acinetobacter sp.
CuSO4
-
Acinetobacter sp.
diethyldithiocarbamate
-
Acinetobacter sp.
dithiothreitol slight Acinetobacter sp.
FeCl3
-
Acinetobacter sp.
FeSO4
-
Acinetobacter sp.
Hg2+ HgCl2 Acinetobacter sp.
KCN
-
Acinetobacter sp.
p-chloromercuribenzoate
-
Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
2,4-Dichlorophenol cosubstrate NADPH Acinetobacter sp.
0.057
-
O2
-
Acinetobacter sp.
0.083
-
NADPH
-
Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
alpha4, 4 * 63000, SDS-PAGE Acinetobacter sp.
240000
-
gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dichlorophenol + NADPH + O2 Acinetobacter sp.
-
3,5-dichlorocatechol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
1.46
-
-
Acinetobacter sp.

Storage Stability

Storage Stability Organism
-20┬░C, 3 months stable Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorophenol + NADPH + O2
-
Acinetobacter sp. 3,5-dichlorocatechol + NADP+ + H2O
-
?
2-chlorophenol + NADPH + O2 incompletely metabolized Acinetobacter sp. ?
-
?
2-cresol + NADPH + O2 incompletely metabolized Acinetobacter sp. ?
-
?
4-bromophenol + NADPH + O2 incompletely metabolized Acinetobacter sp. ?
-
?
4-chloro-2-methylphenol + NADPH + O2
-
Acinetobacter sp. ?
-
?
4-chlorophenol + NADPH + O2
-
Acinetobacter sp. ?
-
?
additional information pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol Acinetobacter sp. ?
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 63000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
29
-
-
Acinetobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.83
-
2,4-Dichlorophenol
-
Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Acinetobacter sp.
FAD FAD cannot be replaced by riboflavin or FMN Acinetobacter sp.
FAD 1 mol of enzyme contains: 1.9 mol FAD Acinetobacter sp.
NADH 5% of the activity with NADPH Acinetobacter sp.
NADPH
-
Acinetobacter sp.