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Literature summary for 1.14.13.2 extracted from

  • Kudryashova, E.V.; Visser, A.J.; van Berkel, W.J.
    Monomer formation and function of p-hydroxybenzoate hydroxylase in reverse micelles and in dimethylsulfoxide/water mixtures (2008), Chembiochem, 9, 413-419.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
4-hydroxybenzoate pH 8, monomeric form Pseudomonas fluorescens
0.025
-
NADPH pH 8, dimeric form Pseudomonas fluorescens
0.025
-
4-hydroxybenzoate pH 8, dimeric form Pseudomonas fluorescens
0.052
-
NADPH pH 8, monomeric form Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
dimer the monomeric form of PHBH, which is not achieved under conventional conditions, is isolated by entrapment in reverse micelles and by addition of DMSO. The PHBH monomer is catalytically more efficient than the PHBH dimer Pseudomonas fluorescens
monomer the monomeric form of PHBH, which is not achieved under conventional conditions, is isolated by entrapment in reverse micelles and by addition of DMSO. The PHBH monomer is catalytically more efficient than the PHBH dimer Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
p-hydroxybenzoate hydroxylase
-
Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45
-
NADPH pH 8, monomeric form Pseudomonas fluorescens
45
-
4-hydroxybenzoate pH 8, monomeric form Pseudomonas fluorescens
63
-
NADPH pH 8, dimeric form Pseudomonas fluorescens
63
-
4-hydroxybenzoate pH 8, dimeric form Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pseudomonas fluorescens