Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion, hanging drops containing 4 mg/ml protein, 100 mM potassium phosphate, pH 7.0, 0.05 mM glutathione, 30 mM sodium sulfite, 0.02 mM FAD, 450 mM ammonium sulfate are equilibrated at 30°C for 7-10 days against a well solution of similar composition, but containing 900 mM ammonium sulfate, crystals of R220Q PHBH diffract to approx. 2.0 A | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
R220Q | 1% of wild-type activity, lower affinity to 4-hydroxybenzoate than wild-type | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | Pseudomonas aeruginosa | - |
protocatechuate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P20586 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHBH | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |