Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | - |
Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
4-hydroxybenzoate | - |
Klebsiella pneumoniae | |
0.025 | - |
NADPH | - |
Klebsiella pneumoniae | |
0.037 | - |
O2 | - |
Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Klebsiella pneumoniae |
80000 | - |
gel filtration | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | Klebsiella pneumoniae | enzyme is expressed at basal level, presence of 4-hydroxybenzoate enhances activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
enzyme is expressed in mutant strain MAO4, but not in wild-type strain | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.76 | - |
- |
Klebsiella pneumoniae |
Storage Stability | Organism |
---|---|
4°C, 50 mM phosphate buffer, 50 mM Tris-HCl, pH 8.0, 10% glycerol, 10 mM EDTA, loss of activity after 72 h | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Klebsiella pneumoniae | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | enzyme is expressed at basal level, presence of 4-hydroxybenzoate enhances activity | Klebsiella pneumoniae | ? | - |
? | |
additional information | a Tyr seems to be involved in substrate activation | Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
50 mM phosphate buffer, 50 mM Tris-HCl, pH 8.0, 10% glycerol, 10 mM EDTA, loss of activity after 72 h | Klebsiella pneumoniae |
21 | - |
room temperature, without glycerol and EDTA, complete inactivation after 20 h | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Klebsiella pneumoniae |