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Literature summary for 1.14.13.2 extracted from

  • Husain, M.; Schopfer, L.M.; Massey, V.
    p-Hydroxybenzoate hydroxylase and melilotate hydroxylase (1978), Methods Enzymol., 53, 543-558.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas fluorescens

General Stability

General Stability Organism
extremely unstable, undergoing rapid inactivation unless protected by substrates and other stabilizing agents Pseudomonas putida
stable to freezing and thawing Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxybenzoate mixed-type Pseudomonas fluorescens
Cl-
-
Delftia acidovorans
Cl-
-
Pseudomonas fluorescens
I-
-
Delftia acidovorans
I-
-
Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
gel filtration Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas putida
-
M-6
-
Pseudomonas putida
-
A 3.12
-
Pseudomonas putida A 3.12
-
A 3.12
-
Pseudomonas putida M-6
-
M-6
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas fluorescens

Reaction

Reaction Comment Organism Reaction ID
4-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O bi uni uni uni ping-pong mechanism Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.5
-
-
Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
4°C, as ammonium sulfate paste, indefinitely stable Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxybenzoate + NADPH + O2 about 1% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
?
3-Fluoro-4-hydroxybenzoate + NADPH + O2 about 1% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
4-aminobenzoate + NADPH + O2 about 1% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas putida protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2
-
Delftia acidovorans protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas putida A 3.12 protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas putida M-6 protocatechuate + NADP+ + H2O
-
?
4-mercaptobenzoate + NADPH + O2 50% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens 4,4'-dithiobisbenzoate + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
50 h, stable Pseudomonas fluorescens
25
-
pH 6.4-8.0, 50 h, stable Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol of FAD per mol of enzyme Pseudomonas fluorescens
NADPH
-
Pseudomonas putida
NADPH
-
Delftia acidovorans
NADPH no activity with NADH Pseudomonas fluorescens