Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
General Stability | Organism |
---|---|
extremely unstable, undergoing rapid inactivation unless protected by substrates and other stabilizing agents | Pseudomonas putida |
stable to freezing and thawing | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxybenzoate | mixed-type | Pseudomonas fluorescens | |
Cl- | - |
Delftia acidovorans | |
Cl- | - |
Pseudomonas fluorescens | |
I- | - |
Delftia acidovorans | |
I- | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas putida | - |
M-6 | - |
Pseudomonas putida | - |
A 3.12 | - |
Pseudomonas putida A 3.12 | - |
A 3.12 | - |
Pseudomonas putida M-6 | - |
M-6 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O | bi uni uni uni ping-pong mechanism | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.5 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
4°C, as ammonium sulfate paste, indefinitely stable | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dihydroxybenzoate + NADPH + O2 | about 1% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O | - |
? | |
3-Fluoro-4-hydroxybenzoate + NADPH + O2 | about 1% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
4-aminobenzoate + NADPH + O2 | about 1% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | ? | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas fluorescens | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas putida | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Delftia acidovorans | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas putida A 3.12 | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas putida M-6 | protocatechuate + NADP+ + H2O | - |
? | |
4-mercaptobenzoate + NADPH + O2 | 50% of the activity with 4-hydroxybenzoate | Pseudomonas fluorescens | 4,4'-dithiobisbenzoate + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
50 h, stable | Pseudomonas fluorescens |
25 | - |
pH 6.4-8.0, 50 h, stable | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol of FAD per mol of enzyme | Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas putida | |
NADPH | - |
Delftia acidovorans | |
NADPH | no activity with NADH | Pseudomonas fluorescens |