Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.195 extracted from

  • Pohlmann, V.; Marahiel, M.A.
    delta-Amino group hydroxylation of L-ornithine during coelichelin biosynthesis (2008), Org. Biomol. Chem., 6, 1843-1848.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cchB, overexpression of N-terminally thioredoxin-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + NADPH + H+ + O2 Streptomyces coelicolor
-
N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 Streptomyces coelicolor A3(2)
-
N5-hydroxy-L-ornithine + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
gene cchB
-
Streptomyces coelicolor A3(2)
-
gene cchB
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
Streptomyces coelicolor

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally thioredoxin-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NADPH + H+ + O2
-
Streptomyces coelicolor N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 absolutely specific for L-ornithine Streptomyces coelicolor N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2
-
Streptomyces coelicolor A3(2) N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 absolutely specific for L-ornithine Streptomyces coelicolor A3(2) N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
additional information no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview Streptomyces coelicolor ?
-
?
additional information no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview Streptomyces coelicolor A3(2) ?
-
?

Synonyms

Synonyms Comment Organism
CchB
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 activity range, pH profile Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces coelicolor
NADPH
-
Streptomyces coelicolor

General Information

General Information Comment Organism
physiological function the enzyme is required for synthesis of the nonproteinogenic amino acids N5-hydroxyornithine and N5-hydroxyformylornithine, that are required for iron assembly by the organism Streptomyces coelicolor