Cloned (Comment) | Organism |
---|---|
overexpression of recombinant enzyme in Escherichia coli both with and without a N-terminal fusion with the maltose binding protein | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
2-heptyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.00013 | - |
2-nonyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.00052 | - |
O2 | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
0.0016 | - |
2-pentyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.014 | - |
2-propyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.032 | - |
NADH | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
0.74 | - |
NADPH | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
x * 86000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-heptyl-4(1H)-quinolone + NADH + H+ + O2 | Pseudomonas aeruginosa | PqsH catalyzes the terminal step in biosynthesis of the Pseudomonas quinolone signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone. Under anaerobic conditions 2-heptyl-3-hydroxy-4(1H)-quinolone is not produced. The enzyme is a biochemical regulator of Pseudomonas quinolone signal controlled social behaviors in Pseudomonas aeruginosa | 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I0Q0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-80°C, purified fusion protein can be stored for one week without loss of enzymatic activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-heptyl-4(1H)-quinolone + NADH + H+ + O2 | PqsH catalyzes the terminal step in biosynthesis of the Pseudomonas quinolone signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone. Under anaerobic conditions 2-heptyl-3-hydroxy-4(1H)-quinolone is not produced. The enzyme is a biochemical regulator of Pseudomonas quinolone signal controlled social behaviors in Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O | - |
? | |
2-heptyl-4(1H)-quinolone + NADH + H+ + O2 | the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons | Pseudomonas aeruginosa | 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O | - |
? | |
2-heptyl-4(1H)-quinolone + NADPH + H+ + O2 | kcat/KM for NADH is 20fold higher than kcat/Km for NADPH | Pseudomonas aeruginosa | 2-heptyl-3-hydroxy-4(1H)-quinolone + NADP+ + H2O | - |
? | |
2-nonyl-4(1H)-quinolone + NADH + H+ + O2 | the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons | Pseudomonas aeruginosa | 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O | - |
? | |
2-pentyl-4(1H)-quinolone + NADH + H+ + O2 | the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons | Pseudomonas aeruginosa | 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O | - |
? | |
2-propyl-4(1H)-quinolone + NADH + H+ + O2 | the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons | Pseudomonas aeruginosa | 3-hydroxy-2-propyl-4(1H)-quinolone + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 86000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
2-heptyl-3,4-dihydroxyquinoline synthase | - |
Pseudomonas aeruginosa |
PqsH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
O2 | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
2.3 | - |
2-propyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
2.5 | - |
2-nonyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
2.7 | - |
2-heptyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
3 | - |
2-pentyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
3.2 | - |
NADH | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
3.8 | - |
NADPH | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | kcat/KM for NADH is 20fold higher than kcat/Km for NADPH | Pseudomonas aeruginosa | |
NADPH | kcat/KM for NADH is 20fold higher than kcat/Km for NADPH | Pseudomonas aeruginosa | |
riboflavin | flavin-dependent monooxygenase, riboflavin is included in the assay | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | PqsH catalyzes the terminal step in biosynthesis of the Pseudomonas quinolone signal molecule 2-heptyl-3,4-dihydroxyquinoline. Under anaerobic conditions 2-heptyl-3-hydroxy-4(1H)-quinolone is not produced. The enzyme is a biochemical regulator of Pseudomonas Quinolone Signal controlled social behaviors in Pseudomonas aeruginosa | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
NADPH | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
17 | - |
2-propyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
100 | - |
NADH | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
190 | - |
2-pentyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
1900 | - |
2-nonyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa | |
2308 | - |
O2 | pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone | Pseudomonas aeruginosa | |
2455 | - |
2-heptyl-4(1H)-quinolone | pH 8.0, 25°C | Pseudomonas aeruginosa |