Cloned (Comment) | Organism |
---|---|
recombinant overexpression of His-tagged enzyme DoxA along with its His-tagged redox partners from Streptomyces peucetius FDX1, FDR2, and FDX3, and the putidaredoxin and putidaredoxin reductase from Pseudomonas putida, that are essential equivalents of the class I type of bacterial electron-transport system, in Escherichia coli strain BL21(DE3) harboring the GroES/EL-expressing plasmid pGro7 | Streptomyces peucetius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
daunorubicin + NADPH + H+ + O2 | Streptomyces peucetius | - |
doxorubicin + NADP+ + H2O | - |
? | |
daunorubicin + NADPH + H+ + O2 | Streptomyces peucetius ATCC 27952 | - |
doxorubicin + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius | - |
- |
- |
Streptomyces peucetius ATCC 27952 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme DoxA from Escherichia coli, copurification with His-tagged FDX1, FDR2, FDX3, and putidaredoxin and putidaredoxin reductase from Pseudomonas putida from Escherichia coli by cobalt affinity chromatography and ultrafiltration | Streptomyces peucetius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
daunorubicin + NADPH + H+ + O2 | - |
Streptomyces peucetius | doxorubicin + NADP+ + H2O | - |
? | |
daunorubicin + NADPH + H+ + O2 | - |
Streptomyces peucetius ATCC 27952 | doxorubicin + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DoxA | - |
Streptomyces peucetius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Streptomyces peucetius | |
NADPH | - |
Streptomyces peucetius |
General Information | Comment | Organism |
---|---|---|
additional information | protein-protein interaction and docking analysis of recombinant enzymes, structure homology modeling, DoxA active site structure, overview. Enzyme DoxA interacts with ist redox partners FDX1, FDR2, and FDX3 | Streptomyces peucetius |
physiological function | the cytochrome P450 monoxygenase DoxA is responsible for the hydroxylation of daunorubicin into doxorubicin | Streptomyces peucetius |