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Literature summary for 1.14.13.18 extracted from

  • Hareland, W.A.; Crawford, R.L.; Chapman, P.J.; Dagley, S.
    Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans (1975), J. Bacteriol., 121, 272-285.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.18

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxyphenylacetic acid
-
 Delftia acidovorans
4-Hydroxy-3-methylphenylacetic acid
-
 Delftia acidovorans
4-hydroxyphenylpropionic acid
-
 Delftia acidovorans
DL-4-hydroxymandelic acid
-
 Delftia acidovorans
KCl
-
 Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 4-hydroxyphenylacetate
-
 Delftia acidovorans
0.067
-
 O2
-
 Delftia acidovorans
0.095
-
 NADH
-
 Delftia acidovorans
0.25
-
 NADPH
-
 Delftia acidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Delftia acidovorans

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Delftia acidovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
-
 Delftia acidovorans

Storage Stability

Storage Stability Organism
4°C, in the presence of dithioerythritol Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-2-methylphenylacetate + NAD(P)H + O2
-
 Delftia acidovorans ?
-
 ?
4-hydroxyhydratropate + NAD(P)H + O2
-
 Delftia acidovorans 2-methylhomogentisate + NAD(P)+ + H2O
-
 ?
4-hydroxyphenoxyacetate + ?
-
 Delftia acidovorans hydroquinone + glycolate + ?
-
 ?
4-hydroxyphenylacetate + NAD(P)H + O2
-
 Delftia acidovorans homogentisate + NAD(P)+ + H2O
-
 ?
4-hydroxyphenylacetate + NADH + H+ + O2
-
 Delftia acidovorans homogentisate + NAD+ + H2O
-
 r
additional information low activity with: 4-hydroxyphenylpropionate, 4-hydroxy-3-methylphenylacetate, 3,4-dihydroxyphenylacetate Delftia acidovorans ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Delftia acidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Delftia acidovorans

Cofactor

Cofactor Comment Organism Structure
FAD FAD required Delftia acidovorans
NADH same maximal velocity with NADH and NADPH Delftia acidovorans
NADPH same maximal velocity with NADH and NADPH Delftia acidovorans