Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
non-heme iron | a non-heme iron oxygenase | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | H9N289 | - |
- |
Pseudomonas putida CBB5 | H9N289 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
caffeine + O2 + NAD(P)H + H+ = theobromine + NAD(P)+ + H2O + formaldehyde | (1) | Pseudomonas putida | |
paraxanthine + O2 + NAD(P)H + H+ = 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | (3) | Pseudomonas putida | |
theophylline + O2 + NAD(P)H + H+ = 3-methylxanthine + NAD(P)+ + H2O + formaldehyde | (2) | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeine + O2 + NAD(P)H + H+ | - |
Pseudomonas putida | theobromine + NAD(P)+ + H2O + formaldehyde | - |
? | |
caffeine + O2 + NAD(P)H + H+ | - |
Pseudomonas putida CBB5 | theobromine + NAD(P)+ + H2O + formaldehyde | - |
? | |
paraxanthine + O2 + NAD(P)H + H+ | - |
Pseudomonas putida | 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
paraxanthine + O2 + NAD(P)H + H+ | - |
Pseudomonas putida CBB5 | 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
theophylline + O2 + NAD(P)H + H+ | - |
Pseudomonas putida | 3-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
theophylline + O2 + NAD(P)H + H+ | - |
Pseudomonas putida CBB5 | 3-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the soluble N-demethylase holoenzyme is composed of two components, a reductase component with cytochrome c reductase activity (Ccr) and a two-subunit N-demethylase component (Ndm). Ndm, with a native molecular mass of 240000 Da, is composed of NdmA (40000 Da) and NdmB (35000 Da). Ccr transfers reducing equivalents from NAD(P)H to Ndm, which catalyses an oxygen-dependent N-demethylation of methylxanthines to xanthine, formaldehyde and water | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ndmA | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is 22% of that with NADH | Pseudomonas putida | |
NADPH | a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is 22% of that with NADH | Pseudomonas putida |