BRENDA - Enzyme Database
show all sequences of 1.14.13.171

Genome mining in Streptomyces avermitilis: A biochemical Baeyer-Villiger reaction and discovery of a new branch of the pentalenolactone family tree

Jiang, J.; Tetzlaff, C.N.; Takamatsu, S.; Iwatsuki, M.; Komatsu, M.; Ikeda, H.; Cane, D.E.; Biochemistry 48, 6431-6440 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces avermitilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
x * 64000, SDS-PAGE
Streptomyces avermitilis
65826
-
x * 65826, predicted from amino acid sequence
Streptomyces avermitilis
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces avermitilis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, butyl-Sepharose column chromatography, gel filtration
Streptomyces avermitilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxy-11-oxopentalenate + NADPH + H+ + O2
-
718853
Streptomyces avermitilis
neopentalenolactone D + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 64000, SDS-PAGE
Streptomyces avermitilis
?
x * 65826, predicted from amino acid sequence
Streptomyces avermitilis
Synonyms
Synonyms
Commentary
Organism
ptlE
-
Streptomyces avermitilis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Streptomyces avermitilis
NADPH
-
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces avermitilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Streptomyces avermitilis
NADPH
-
Streptomyces avermitilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
x * 64000, SDS-PAGE
Streptomyces avermitilis
65826
-
x * 65826, predicted from amino acid sequence
Streptomyces avermitilis
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, butyl-Sepharose column chromatography, gel filtration
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxy-11-oxopentalenate + NADPH + H+ + O2
-
718853
Streptomyces avermitilis
neopentalenolactone D + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 64000, SDS-PAGE
Streptomyces avermitilis
?
x * 65826, predicted from amino acid sequence
Streptomyces avermitilis
Other publictions for EC 1.14.13.171
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718892
Seo
Genome mining in Streptomyces. ...
Streptomyces arenae, Streptomyces avermitilis, Streptomyces exfoliatus, Streptomyces exfoliatus UC5319, Streptomyces arenae TUE469
Biochemistry
50
1739-1754
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
6
-
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
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-
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6
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
718853
Jiang
Genome mining in Streptomyces ...
Streptomyces avermitilis
Biochemistry
48
6431-6440
2009
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1
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2
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1
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1
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1
2
1
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2
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1
2
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2
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1
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1
2
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