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Literature summary for 1.14.13.168 extracted from

  • Dai, X.; Mashiguchi, K.; Chen, Q.; Kasahara, H.; Kamiya, Y.; Ojha, S.; Dubois, J.; Ballou, D.; Zhao, Y.
    The biochemical mechanism of auxin biosynthesis by an Arabidopsis YUCCA flavin-containing monooxygenase (2013), J. Biol. Chem., 288, 1448-1457.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.168

Cloned(Commentary)

Cloned (Comment) Organism
expession in Escherichia coli Arabidopsis thaliana
expressed in Escherichia coli BL21 Star (DE3) cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 NADPH in the presence of phenylpyruvate, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C Arabidopsis thaliana
0.043
-
 phenylpyruvate in the presence of NADPH, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, isoform YUC6, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q8VZ59
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)pyruvate + NADPH + H+ + O2
-
 Arabidopsis thaliana (indol-3-yl)acetate + NADP+ + H2O + CO2
-
 ?
(indol-3-yl)pyruvate + NADPH + H+ + O2
-
 Arabidopsis thaliana (indol-3-yl)acetate + NADP+ + H2O + CO2 first step of the YUC6-catalyzed reaction is the reduction of the FAD cofactor to FADH- by NADPH. Subsequently, FADH- reacts with oxygen to form a flavin-C4a-(hydro)peroxy intermediate, which has a maximum absorbance at 381 nm in its UV-visible spectrum. The final chemical step is the reaction of the C4a-intermediate with (indol-3-yl)pyruvate to produce (indol-3-yl)acetate. The YUC6 intermediate has a half-life of about 20 s ?
phenylpyruvate + NADPH + H+ + O2 isoform YUC6 shows basal NADPH oxidase activity in the absence of the cosubstrate phenylpyruvate Arabidopsis thaliana phenylacetate + NADP+ + H2O + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, isoform YUC6, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At5g25620 locus name Arabidopsis thaliana
YUC6
-
 Arabidopsis thaliana
YUC6 isoform Arabidopsis thaliana
YUCCA
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
 NADPH in the presence of phenylpyruvate, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arabidopsis thaliana
FAD isoform YUC6 contains oxidized flavin as a cofactor Arabidopsis thaliana
additional information isoform YUC6 does not accept FMN as cofactor Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the enzyme catalyzes a rate-limiting step in auxin biosynthesis and is essential for many developmental processes Arabidopsis thaliana