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Literature summary for 1.14.13.167 extracted from

  • Min, J.; Lu, Y.; Hu, X.; Zhou, N.Y.
    Biochemical characterization of 3-methyl-4-nitrophenol degradation in Burkholderia sp. strain SJ98 (2016), Front. Microbiol., 7, 791 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.167

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme is encoded in the pnpABA1CDEF cluster, which is involved in para-nitrophenol (PNP) and 2-chloro-4-nitrophenol (2C4NP) catabolism and is also likely responsible for 3-methyl-4-nitrophenol (3M4NP) degradation in strain SJ98, real-time quantitative PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS Burkholderia sp.

Protein Variants

Protein Variants Comment Organism
additional information generation of pnpA deletion mutant SJ981pnpA1, the mutant shows that pnpA is absolutely necessary for the catabolism of 3-methyl-4-nitrophenol Burkholderia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Burkholderia sp.
0.0203
-
 3-Methyl-4-nitrophenol pH and temperature not specified in the publication Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methyl-4-nitrophenol + NADPH + H+ + O2 Burkholderia sp.
-
 2-methyl-1,4-benzoquinone + nitrite + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme PnpA from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-4-nitrophenol + NADPH + H+ + O2
-
 Burkholderia sp. 2-methyl-1,4-benzoquinone + nitrite + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
PNP 4-monooxygenase
-
 Burkholderia sp.
pnpA
-
 Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Burkholderia sp.
NADPH
-
 Burkholderia sp.

General Information

General Information Comment Organism
metabolism the enzyme is involved in 3-methyl-4-nitrophenol degradation in Burkholderia sp. strain SJ98, phathway overview. Methyl-1,4-benzoquinone (MBQ) and methylhydroquinone (MHQ), rather than catechol proposed previously, are identified as the intermediates before ring cleavage during 3-methyl-4-nitrophenol degradation by Burkholderia sp. strain SJ98. By sequential catalysis assays, PnpCD, PnpE, and PnpF are likely involved in the further pathway of 3-methyl-4-nitrophenol catabolism Burkholderia sp.
physiological function PnpA, a PNP 4-monooxygenase, is able to catalyze the monooxygenation of 3-methyl-4-nitrophenol to 3-methyl-1,4-benzoquinone. PnpB, a 1,4-benzoquinone reductase, has the ability to catalyze the reduction of 3-methyl-1,4-benzoquinone to methylhydroquinone. Moreover, PnpB is also able to enhance PnpA activity in vitro in the conversion of 3-methyl-4-nitrophenol to 3-methyl-1,4-benzoquinone Burkholderia sp.