Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Rhodococcus opacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59700 | - |
x * 59700, calculated from amino acid sequence | Rhodococcus opacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | - |
- |
- |
Rhodococcus opacus SAO101 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-nitrophenol-grown cell | - |
Rhodococcus opacus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000046 | - |
using 2,4,6-trinitrocatechol as substrate, pH and temperature not specified in the publication | Rhodococcus opacus |
0.000025 | - |
using 4-nitrocatechol as substrate, pH and temperature not specified in the publication | Rhodococcus opacus |
0.0000264 | - |
using 4-nitrophenol as substrate, pH and temperature not specified in the publication | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trinitrophenol + NADH + H+ + O2 | 17% activity compared to 4-nitrophenol | Rhodococcus opacus | ? | - |
? | |
2,4,6-trinitrophenol + NADH + H+ + O2 | 17% activity compared to 4-nitrophenol | Rhodococcus opacus SAO101 | ? | - |
? | |
4-nitrocatechol + NADH + H+ + O2 | 96% activity compared to 4-nitrophenol | Rhodococcus opacus | 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O | - |
? | |
4-nitrocatechol + NADH + H+ + O2 | 96% activity compared to 4-nitrophenol | Rhodococcus opacus SAO101 | 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O | - |
? | |
4-nitrophenol + NADH + H+ + O2 | 100% activity | Rhodococcus opacus | ? | - |
? | |
4-nitrophenol + NADH + H+ + O2 | 100% activity | Rhodococcus opacus SAO101 | ? | - |
? | |
additional information | almost no activity towards 2,4,6-trinitrophenol | Rhodococcus opacus | ? | - |
? | |
additional information | almost no activity towards 2,4,6-trinitrophenol | Rhodococcus opacus SAO101 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59700, calculated from amino acid sequence | Rhodococcus opacus |
Synonyms | Comment | Organism |
---|---|---|
4-NP/4-NCA monooxygenase | - |
Rhodococcus opacus |
NpcA | - |
Rhodococcus opacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodococcus opacus |
General Information | Comment | Organism |
---|---|---|
malfunction | the npcA mutant strain SDA1 completely loses the ability to grow on 4-nitrophenol as the sole carbon source | Rhodococcus opacus |