BRENDA - Enzyme Database
show all sequences of 1.14.13.166

Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3

Zhang, J.J.; Liu, H.; Xiao, Y.; Zhang, X.E.; Zhou, N.Y.; J. Bacteriol. 191, 2703-2710 (2009) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
addition of Mg2+ or Mn2+ ions has no effect on the activity
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
1 * 47000, His-tagged enzyme, gel filtration
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
C1I201
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:4-nitrophenol-grown cell
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
at pH 7.0 and 30°C
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20°C, without glycerol, rapid inactivation
Pseudomonas sp.
-70°C, in 50% (v/v) glycerol, 7 days, 70% loss of activity
Pseudomonas sp.
0°C, storage medium, no loss of activity after several hours, 30% loss of activity after 12 h, 80% loss of activity after 40 h
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrocatechol + NADPH + H+ + O2
-
704288
Pseudomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 47000, His-tagged enzyme, gel filtration
Pseudomonas sp.
Synonyms
Synonyms
Commentary
Organism
para-nitrophenol 4-monooxygenase
-
Pseudomonas sp.
PNP 4-monooxygenase
-
Pseudomonas sp.
pnpA
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
dependent on, negligible activity is observed when FMN replaces FAD
Pseudomonas sp.
NADH
NADPH can be replaced by NADH with the same efficiency
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
dependent on, negligible activity is observed when FMN replaces FAD
Pseudomonas sp.
NADH
NADPH can be replaced by NADH with the same efficiency
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
addition of Mg2+ or Mn2+ ions has no effect on the activity
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
1 * 47000, His-tagged enzyme, gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:4-nitrophenol-grown cell
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
at pH 7.0 and 30°C
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, without glycerol, rapid inactivation
Pseudomonas sp.
-70°C, in 50% (v/v) glycerol, 7 days, 70% loss of activity
Pseudomonas sp.
0°C, storage medium, no loss of activity after several hours, 30% loss of activity after 12 h, 80% loss of activity after 40 h
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrocatechol + NADPH + H+ + O2
-
704288
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 47000, His-tagged enzyme, gel filtration
Pseudomonas sp.
Other publictions for EC 1.14.13.166
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744109
Min
A two-component para-nitrophe ...
no activity in Rhodococcus imtechensis
Appl. Environ. Microbiol.
82
714-723
2016
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1
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-
-
-
-
-
-
745126
Kallubai
In silico approach to support ...
Arthrobacter sp. JS443, Lysinibacillus sphaericus, Lysinibacillus sphaericus JS905
Interdiscip. Sci. Comput. Life Sci.
7
157-167
2015
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-
-
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-
-
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3
-
8
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6
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8
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4
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4
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3
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6
-
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7
7
-
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp., Pseudomonas sp. 1-7
BMC Microbiol.
12
27
2012
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8
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2
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3
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2
-
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711336
Wei
para-Nitrophenol 4-monooxygena ...
Pseudomonas sp.
Biodegradation
21
915-921
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
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-
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1
-
3
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1
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1
1
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1
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1
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-
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1
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-
1
-
-
704288
Zhang
Identification and characteriz ...
Pseudomonas sp.
J. Bacteriol.
191
2703-2710
2009
-
-
1
-
-
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-
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-
1
1
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-
6
-
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1
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-
1
1
3
1
1
3
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3
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-
1
3
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1
1
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1
-
1
1
3
1
1
-
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-
718490
Liu
Expression, purification, crys ...
Pseudomonas putida, Pseudomonas putida DLL-E4
Acta Crystallogr. Sect. F
65
1004-1006
2009
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-
1
1
-
-
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1
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5
-
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1
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2
1
3
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2
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1
2
1
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1
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1
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2
1
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-
719711
Perry
Cloning of a gene cluster invo ...
Arthrobacter sp.
J. Bacteriol.
189
7563-7572
2007
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1
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-
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-
-
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7
-
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1
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1
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15
-
2
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2
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1
2
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1
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1
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15
-
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-
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-
-
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-
438817
Leung
The role of the Sphingomonas s ...
Sphingomonas sp., Sphingomonas sp. UG30
FEMS Microbiol. Lett.
173
247-253
1999
-
-
-
-
-
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1
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2
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2
1
1
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1
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1
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1
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2
1
-
-
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-
-
-
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-
-
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-
438885
Kadiyala
A two-component monooxygenase ...
Lysinibacillus sphaericus, Lysinibacillus sphaericus JS905
Appl. Environ. Microbiol.
64
2479-2484
1998
-
-
-
-
-
-
3
-
-
1
-
-
-
3
-
-
1
-
-
1
4
2
3
-
1
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1
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1
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1
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3
-
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1
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1
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1
4
2
3
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1
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