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Literature summary for 1.14.13.161 extracted from
- Induction and characterization of a cytochrome P-450-dependent camphor hydroxylase in tissue cultures of common sage (Salvia officinalis) (1993), Plant Physiol., 101, 1231-1237.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FAD | inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold | Salvia officinalis |  |
| FMN | inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold | Salvia officinalis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| clotrimazole | 84% inhibition at 0.05 mM | Salvia officinalis | |
| CO | 73% inhibition at CO:O2 ratio of 9:1, activity is partially restored by blue light | Salvia officinalis | |
| cytochrome c | 95% inhibition at 0.05 mM | Salvia officinalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
(+)-Camphor | pH 7.0, 32°C | Salvia officinalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | microsomal membrane | Salvia officinalis | 16020 | - |
| microsome | microsomal membrane | Salvia officinalis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MnCl2 | upon treatment of sage suspension cultures with 30 mM MnCI2, camphor-6-hydroxylase activity is induced up to 7fold | Salvia officinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58000, SDS-PAGE | Salvia officinalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salvia officinalis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Salvia officinalis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + NADPH + H+ + O2 | - |
Salvia officinalis | (+)-6-exo-hydroxycamphor + NADP+ + H2O | - |
? | |
| additional information | the microsomal camphor-6-exo-hydroxylase system requires molecular oxygen and a reduced pyridine nucleotide | Salvia officinalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Salvia officinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Salvia officinalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | more than 60% of maximum activity within | Salvia officinalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 21% of the rate with NADPH | Salvia officinalis | |
| NADPH | - |
Salvia officinalis | |
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