Literature summary for 1.14.13.161 extracted from

Kim, D.; Ortiz de Montellano, P.R.
Tricistronic overexpression of cytochrome P450cam, putidaredoxin, and putidaredoxin reductase provides a useful cell-based catalytic system (2009), Biotechnol. Lett., 31, 1427-1431.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-camphor + NADPH + H+ + O2	-	Pseudomonas putida	(+)-6-exo-hydroxycamphor + NADP+ + H2O	-	?
limonene + NADPH + H+ + O2	-	Pseudomonas putida	(-)-perillyl alcohol + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
P450cam	-	Pseudomonas putida

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Release 2023.1 (January 2023)