BRENDA - Enzyme Database
show all sequences of 1.14.13.161

Tricistronic overexpression of cytochrome P450cam, putidaredoxin, and putidaredoxin reductase provides a useful cell-based catalytic system

Kim, D.; Ortiz de Montellano, P.R.; Biotechnol. Lett. 31, 1427-1431 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pseudomonas putida
Engineering
Protein Variants
Commentary
Organism
additional information
functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(+)-camphor + NADPH + H+ + O2
-
702820
Pseudomonas putida
(+)-6-exo-hydroxycamphor + NADP+ + H2O
-
-
-
?
limonene + NADPH + H+ + O2
-
702820
Pseudomonas putida
(-)-perillyl alcohol + NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
P450cam
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(+)-camphor + NADPH + H+ + O2
-
702820
Pseudomonas putida
(+)-6-exo-hydroxycamphor + NADP+ + H2O
-
-
-
?
limonene + NADPH + H+ + O2
-
702820
Pseudomonas putida
(-)-perillyl alcohol + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.161
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
702820
Kim
Tricistronic overexpression of ...
Pseudomonas putida
Biotechnol. Lett.
31
1427-1431
2009
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718227
Funk
Induction and characterization ...
Salvia officinalis
Plant Physiol.
101
1231-1237
1993
2
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1
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717146
Funk
Catabolism of camphor in tissu ...
Salvia officinalis
Arch. Biochem. Biophys.
294
306-313
1992
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717740
Koga
Cloning and nucleotide sequenc ...
Pseudomonas putida
J. Biochem.
106
831-836
1989
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