Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.160 extracted from

  • Kadow, M.; Loschinski, K.; Sass, S.; Schmidt, M.; Bornscheuer, U.
    Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization (2012), Appl. Microbiol. Biotechnol., 96, 419-429.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one pH 9.0, 20°C Pseudomonas putida
0.127
-
Cyclobutanone pH 9.0, 20°C Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein Pseudomonas putida
63500
-
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida H3JQW0
-
-
Pseudomonas putida NCIMB 10007 H3JQW0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2200
-
pH 9.0, 20°C Pseudomonas putida

Storage Stability

Storage Stability Organism
4°C, 24 h, 50% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+
-
Pseudomonas putida ? + NADP+ + H2O
-
?
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+
-
Pseudomonas putida NCIMB 10007 ? + NADP+ + H2O
-
?
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+ 34% conversion Pseudomonas putida ? + NADP+ + H2O
-
?
2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+ 34% conversion Pseudomonas putida NCIMB 10007 ? + NADP+ + H2O
-
?
2-cyclohexen-1-one + O2 + NADPH + H+ 74% conversion Pseudomonas putida ? + NADP+ + H2O
-
?
2-cyclohexen-1-one + O2 + NADPH + H+ 74% conversion Pseudomonas putida NCIMB 10007 ? + NADP+ + H2O
-
?
2-cyclopenten-1-one + O2 + NADPH + H+ 62% conversion Pseudomonas putida ? + NADP+ + H2O
-
?
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+ 22% conversion Pseudomonas putida ? + NADP+ + H2O
-
?
3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+ 22% conversion Pseudomonas putida NCIMB 10007 ? + NADP+ + H2O
-
?
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+ 13% conversion Pseudomonas putida ? + NADP+ + H2O
-
?
3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+ 13% conversion Pseudomonas putida NCIMB 10007 ? + NADP+ + H2O
-
?
3-methyl-2-cyclopenten-1-one + O2 + NADPH + H+ 13% conversion Pseudomonas putida ? + NADP+ + H2O
-
?
cyclobutanone + O2 + NADPH + H+
-
Pseudomonas putida ? + NADP+ + H2O
-
?
norcamphor + O2 + NADPH + H+
-
Pseudomonas putida ? + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
half-life 196 min Pseudomonas putida
25
-
half-life 43 min Pseudomonas putida
30
-
half-life 9 min Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.7
-
Cyclobutanone pH 9.0, 20°C Pseudomonas putida
49.3
-
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one pH 9.0, 20°C Pseudomonas putida