Literature summary for 1.14.13.149 extracted from

Teufel, R.; Mascaraque, V.; Ismail, W.; Voss, M.; Perera, J.; Eisenreich, W.; Haehnel, W.; Fuchs, G.
Bacterial phenylalanine and phenylacetate catabolic pathway revealed (2010), Proc. Natl. Acad. Sci. USA, 107, 14390-14395.

Search for more literature for 1.14.13.149

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	maltose-binding protein-tagged PaaD added separately does not affect the specific activity of PaaABCE significantly	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pseudomonas sp. strain Y2	Escherichia coli

General Stability

General Stability	Organism
the ability of PaaABC(D)E to oxygenate its substrate is largely lost within 5 min in an enzymatic assay (50 mMTris-HCl (pH 8.0) at 30°C)	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
amylose resin column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phenylacetyl-CoA + O2 + NADPH + H+	-	Escherichia coli	2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+	-	ir

Synonyms

Synonyms	Comment	Organism
PaaABCDE	-	Escherichia coli
PaaAC(D)	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)