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Literature summary for 1.14.13.148 extracted from

  • Wang, X.; Zhang, N.; Teng, Z.; Wang, P.; Zhang, W.; Chen, X.; Zhang, Y.; Chen, Y.; Fu, H.; Li, C.
    Structural and mechanistic insights into dimethylsulfoxide formation through dimethylsulfide oxidation (2021), Front. Microbiol., 12, 735793 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.148

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of Tmm and Tmm soaked with dimethylsulfide. The binding of dimethylsulfide may repel the nicotinamide ring of NADP+, and make NADP+ generate a conformational change, shutting off the substrate entrance and exposing the active C4a-(hydro)peroxyflavin to dimethylsulfide to complete the oxidation Candidatus Pelagibacter sp. HTCC7211

Protein Variants

Protein Variants Comment Organism
D314A mutation decreases the activity significantly Candidatus Pelagibacter sp. HTCC7211
D314E enzymatic activity and the apparent KM toward NADPH are only slightly affected Candidatus Pelagibacter sp. HTCC7211
H227A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
N288A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
N72A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
R226A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
R409A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
S203A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
 NADPH mutant D314E, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0128
-
 NADPH wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.032
-
 NADPH mutant D314A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0341
-
 NADPH mutant N72A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0412
-
 NADPH mutant H227A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0449
-
 NADPH mutant R409A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0611
-
 NADPH mutant N288A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.1018
-
 NADPH mutant R226A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.116
-
 Methimazole wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.1348
-
 NADPH mutant S203A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.139
-
 N,N,N-trimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.181
-
 N,N-dimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.25
-
 Dimethyl sulfide wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211

Organism

Organism UniProt Comment Textmining
Candidatus Pelagibacter sp. HTCC7211 B6BT33 dimethyl sulfoxide/trimethylamine N-oxide reductase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethyl sulfide + NADPH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 dimethyl sulfoxide + NADP+ + H2O
-
 ?
methimazole + NADPH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 ? + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 N,N,N-trimethylamine N-oxide + NAD+ + H2O
-
 ?
N,N-dimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 N,N-dimethylamine N-oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
dimethyl sulfoxide/trimethylamine N-oxide reductase
-
 Candidatus Pelagibacter sp. HTCC7211
PB7211
-
 Candidatus Pelagibacter sp. HTCC7211

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 NADPH mutant R226A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0167
-
 NADPH mutant S203A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0217
-
 NADPH mutant N288A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0317
-
 NADPH mutant N72A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.035
-
 NADPH mutant D314A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0417
-
 NADPH mutant H227A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.045
-
 NADPH mutant R409A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.075
-
 NADPH wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.075
-
 Dimethyl sulfide wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.0867
-
 NADPH mutant D314E, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.087
-
 Methimazole wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.298
-
 N,N-dimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
0.373
-
 N,N,N-trimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211

Cofactor

Cofactor Comment Organism Structure
FAD FAD and NADPH molecules are essential for the catalysis Candidatus Pelagibacter sp. HTCC7211
NADPH FAD and NADPH molecules are essential for the catalysis Candidatus Pelagibacter sp. HTCC7211

General Information

General Information Comment Organism
metabolism the catalytic mechanism comprises a reductive half-reaction and an oxidative half-reaction. In the reductive half-reaction, FAD is reduced by NADPH. In the oxidative half-reaction, the reduced FAD reacts with O2 to form the C4a-(hydro)peroxyflavin Candidatus Pelagibacter sp. HTCC7211

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
 Dimethyl sulfide wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.64
-
 N,N-dimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
2.68
-
 N,N,N-trimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211